A brain-enriched Drp1 isoform associates with lysosomes, late endosomes, and the plasma membrane

The Journal of Biological Chemistry
Kie ItohHiromi Sesaki

Abstract

Dynamin-related protein 1 (Drp1) constricts mitochondria as a mechanochemical GTPase during mitochondrial division. The Drp1 gene contains several alternative exons and produces multiple isoforms through RNA splicing. Here we performed a systematic analysis of Drp1 transcripts in different mouse tissues and identified a previously uncharacterized isoform that is highly enriched in the brain. This Drp1 isoform is termed Drp1ABCD because it contains four alterative exons: A, B, C, and D. Remarkably, Drp1ABCD is located at lysosomes, late endosomes, and the plasma membrane in addition to mitochondria. Furthermore, Drp1ABCD is concentrated at the interorganelle interface between mitochondria and lysosomes/late endosomes. The localizations of Drp1ABCD at lysosomes, late endosomes, and the plasma membrane require two exons, A and B, that are present in the GTPase domain. Drp1ABCD assembles onto these membranes in a manner that is regulated by its oligomerization and GTP hydrolysis. Experiments using lysosomal inhibitors show that the association of Drp1ABCD with lysosomes/late endosomes depends on lysosomal pH but not their protease activities. Thus, Drp1 may connect mitochondria to endosomal-lysosomal pathways in addition to mitocho...Continue Reading

References

Sep 16, 2009·The Journal of Cell Biology·Junko WakabayashiHiromi Sesaki
Jul 24, 2010·Annals of the New York Academy of Sciences·Chuang-Rung Chang, Craig Blackstone
May 14, 2011·Current Opinion in Cell Biology·Yusuke KageyamaHiromi Sesaki
Jun 28, 2011·Cell·Yasushi TamuraHiromi Sesaki
May 9, 2012·The Journal of Cell Biology·Yusuke KageyamaHiromi Sesaki
Jun 12, 2012·Biochimica Et Biophysica Acta·Timothy E Shutt, Heidi M McBride
Jun 27, 2013·The Journal of Cell Biology·Stefan StrackJ Thomas Cribbs
Jan 15, 2014·The International Journal of Biochemistry & Cell Biology·Ronald A Merrill, Stefan Strack
Jan 17, 2014·Nature·Jonathan R Friedman, Jodi Nunnari
Jul 19, 2014·PloS One·Itsasne Bustillo-ZabalbeitiaJean-Claude Martinou
Oct 22, 2014·Circulation Research·Yoshiyuki IkedaJunichi Sadoshima
Feb 7, 2015·Molecular Cell·Jennifer A KashatusDavid F Kashatus
Feb 24, 2015·Current Opinion in Cell Biology·Madhuparna RoyHiromi Sesaki
Mar 15, 2015·The Journal of Biological Chemistry·Christopher A FrancyJason A Mears
Sep 17, 2016·Molecular Cell·Yoshihiro AdachiHiromi Sesaki
Sep 16, 2017·Trends in Cell Biology·Shoichiro KameokaHiromi Sesaki
Feb 6, 2018·Journal of Cellular and Molecular Medicine·Priya GajendiranShanmugasundaram Ganapathy-Kanniappan

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Citations

Dec 5, 2019·Journal of Biochemistry·Daisuke MurataHiromi Sesaki
May 23, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mark A TarnopolskyBekim Sadikovic
Jul 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Wesley PengDimitri Krainc
Oct 31, 2018·Journal of Cell Science·Qian Feng, Benoît Kornmann
Aug 17, 2019·EMBO Reports·Isabel Gordaliza-AlagueroAntonio Zorzano
Jan 16, 2021·Trends in Cell Biology·Luis-Carlos TábaraJulien Prudent
Feb 5, 2021·Nature·Felix KrausMichael T Ryan
Jun 1, 2020·Pharmacology & Therapeutics·Ayeshah A RosdahShiang Y Lim

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