A brief practical review of size exclusion chromatography: Rules of thumb, limitations, and troubleshooting

Protein Expression and Purification
Richard R Burgess

Abstract

Through years of research, teaching, troubleshooting, and editing, I have developed a solid working understanding of many aspects of protein purification and protein biochemistry. I have also found that many researchers do not understand the basics, let alone the pitfalls, of the techniques they use. In the case of size exclusion chromatography (SEC), I summarize below some of the rules of thumb, suggestions, limitations and troubleshooting I have found most useful in helping my students and colleagues carry out successful SEC.

Citations

Sep 4, 2020·Current Protocols in Protein Science·Christina J WelchTarun K Dam
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Nagesh K Tripathi, Ambuj Shrivastava
Aug 9, 2020·Frontiers in Genetics·Eren M Veziroglu, George I Mias
Apr 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nicola Volpi
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Pei JiangRenjie Chai
Dec 18, 2020·Frontiers in Molecular Neuroscience·Pedro M MartinsSandra Macedo-Ribeiro
Sep 18, 2020·Advances in Colloid and Interface Science·Shixiang LiuHailin Cong
Oct 16, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jin WangEmmanuel A Ho
Sep 11, 2019·Journal of Pharmaceutical and Biomedical Analysis·Kaleb J DuelgeMichael R Zachariah
Feb 19, 2021·Frontiers in Physiology·Joshua P NederveenMark A Tarnopolsky
Feb 21, 2021·Analytica Chimica Acta·Ester LubomirskyR Dario Arrua
Oct 14, 2021·Biochemical Society Transactions·Stephanie A NestorowVerna Frasca
May 16, 2020·Food Chemistry·Johana P Márquez-LázaroFidel Toldrá

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