A broadband capacitive sensing method for label-free bacterial LPS detection

Biosensors & Bioelectronics
Artur RydoszSlawomir Gruszczynski

Abstract

In this paper, the authors present a new type of highly sensitive label-free microwave sensor in a form of interdigital capacitor coated with T4 bacteriophage gp37 adhesin. The adhesin binds Escherichia coli B (E. coli B) by precise recognizing its bacterial host lipopolysaccharide (LPS). The C-terminal part of the adhesin consists of the receptor-binding amino acid residues which are involved in a specific interaction with two terminal glucose residues of the bacterial LPS. The change of the sensors' capacitance and conductance as a subject to LPS presence is an indicator of the detection. The measurements in the frequency range of 0-3GHz utilizing vector network analyzer have been carried out at different concentrations to verify experimentally the proposed method. The measured capacitance change between the reference and the biofunctionalized sensor equals 15% in the entire frequency range and the measured conductance change exceeds 19%. The changes of both parameters can be used as good indicators of the LPS detection. The selectivity has been confirmed by the ELISA experiments and tested by sensor measurements with lipopolysaccharide (LPS) from E. coli B, E. coli 056, E. coli 0111, Pseudomonas aeruginosa NBRC 13743 and Haf...Continue Reading

References

Mar 31, 2005·Biosensors & Bioelectronics·Joon Myong SongTuan Vo-Dinh
Jun 30, 2006·Biosensors & Bioelectronics·Dai KatoOsamu Niwa
Dec 2, 2008·Biosensors & Bioelectronics·Madhukar Varshney, Yanbin Li
Dec 6, 2011·Biosensors & Bioelectronics·Melinda S McClellanRyan C Bailey
Feb 7, 2012·Diabetes Research and Clinical Practice·Seungwan KimKiejin Lee
Nov 26, 2013·Biosensors & Bioelectronics·Hyunggoo ParkSeong Chan Jun
Dec 3, 2013·Biosensors & Bioelectronics·Sarra El IchiChantal Fournier-Wirth
Jul 30, 2014·Biosensors & Bioelectronics·Ewa BrzozowskaWojtek J Bock
Dec 3, 2014·Biosensors & Bioelectronics·Rasheeda M Hawk, Andrea M Armani
May 30, 2015·Biosensors & Bioelectronics·Miguel A D NevesMichael Thompson

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Citations

Feb 22, 2017·Sensors·Gizem Ertürk, Bo Mattiasson
May 3, 2019·Reviews in Medical Virology·Sana RehmanSaadia Naseem
Jun 16, 2018·Journal of Agricultural and Food Chemistry·Jiadi SunXiulan Sun
Oct 8, 2021·ACS Infectious Diseases·Catarina L NogueiraCarla M Carvalho

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