PMID: 9421516Feb 28, 1998Paper

A broader role for AU-rich element-mediated mRNA turnover revealed by a new transcriptional pulse strategy

Nucleic Acids Research
Nianhua XuA B Shyu

Abstract

The widespread occurrence of AU-rich elements (AREs) in mRNAs encoding proteins with diversified functions and synthesized under a vast variety of physiological conditions suggests that AREs are involved in finely tuned and stringent control of gene expression. Thus it is important to investigate the regulation of ARE-mediated mRNA decay in a variety of mammalian cells in different physiological states. The tetracycline (Tet)-regulatory promoter system appears appropriate for these investigations. However, we found that efficient degradation of mRNAs bearing different AREs cannot be observed simply by blocking constitutive transcription from the Tet-regulated promoter with Tet, possibly due to saturation of the cellular decay machinery. In addition, deadenylation kinetics and their relationship to mRNA decay cannot be adequately measured under these conditions. To overcome these obstacles we have developed a new strategy that employs the Tet-regulated promoter system to achieve a transient burst of transcription that results in synthesis of a population of cytoplasmic mRNAs fairly homogeneous in size. Using this new system we show that ARE-destabilizing function, necessary for down-regulating mRNAs for cytokines, growth factors...Continue Reading

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Citations

Mar 8, 2000·International Journal for Parasitology·J K Casinader SaverimuttuS M Jayasena
Oct 20, 2009·Nature Structural & Molecular Biology·Chyi-Ying A ChenAnn-Bin Shyu
Nov 26, 2002·European Journal of Biochemistry·Frank BolligHelmut Holtmann
Jun 6, 2012·The Journal of Biological Chemistry·Guoan HeAihao Ding
May 5, 2009·Molecular & Cellular Proteomics : MCP·Benoît LalooChristophe Grosset
Feb 11, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Angels NavarroAndrew C Larner
Aug 29, 2002·The EMBO Journal·Georg StoecklinChristoph Moroni
Jan 27, 2004·Nucleic Acids Research·P Couttet, T Grange
Jan 5, 2006·Nucleic Acids Research·Carine BarreauH Beverley Osborne
Jul 15, 2009·Nucleic Acids Research·Angela SchwedeChristine Clayton
Nov 20, 2012·Nucleic Acids Research·Christina EhlersJan Chemnitz
Feb 18, 2005·Nucleic Acids Research·Dominic C VoonJohn M Daly
Jun 9, 2001·Journal of Pediatric Gastroenterology and Nutrition·B L Shneider
May 21, 2008·Molecular and Cellular Biology·Yuki KuwanoMyriam Gorospe
Nov 17, 2010·Molecular and Cellular Biology·Sandra L ClementJens Lykke-Andersen
Sep 21, 2002·Molecular and Cellular Biology·Chyi-Ying A ChenAnn-Bin Shyu
Aug 29, 2003·Molecular and Cellular Biology·Bedabrata SarkarRobert J Schneider
Jan 31, 2003·Molecular and Cellular Biology·Jian KongStephen A Liebhaber
Mar 1, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Helen W HuangJoseph L Alcorn
Mar 15, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ceá C TillisJoseph L Alcorn
Nov 17, 2007·American Journal of Respiratory Cell and Molecular Biology·Helen W HuangJoseph L Alcorn

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