PMID: 9429252Jan 16, 1998Paper

A Brucella melitensis high-temperature-requirement A (htrA) deletion mutant is attenuated in goats and protects against abortion

Research in Veterinary Science
R W PhillipsR Martin Roop

Abstract

It has been previously demonstrated that a Brucella melitensis high-temperature-requirement A (htrA) deletion mutant is more susceptible to oxidative killing in vitro than the parental strain and is attenuated in mice. To evaluate the contribution of the B melitensis HtrA protease to virulence in ruminants, the capacity of the B melitensis htrA mutant RWP5 to produce abortion in goats was compared to that of the virulent parental strain 16M. Experimental infection with strain 16M caused abortion in eight of 12 pregnant nannies, while none of the 12 nannies inoculated with RWP5 aborted. Furthermore, intramuscular injection of fetuses in utero with RWP5 led to colonisation of the fetus with subsequent colonisation of the nanny, but no abortion was observed. Nannies vaccinated with RWP5 showed complete protection against abortion when challenged with 16M during the third trimester of pregnancy. However, these animals were not protected from colonisation by 16M. The results presented here clearly indicate that the B melitensis htrA gene product contributes to pathogenesis in goats, but the utility of B melitensis htrA mutants as vaccines in this host appears to be limited.

References

Feb 1, 1991·Molecular Microbiology·K JohnsonC Hormaeche
Feb 1, 1990·The Journal of Antimicrobial Chemotherapy·M B al-Sibai, S M Qadri

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Citations

Jul 18, 2001·Vaccine·R W Titball, E D Williamson
Nov 5, 2002·Veterinary Microbiology·Axel CloeckaertPhilip H Elzer
Nov 5, 2002·Veterinary Microbiology·P H ElzerF M Enright
Jun 23, 2006·Infection and Immunity·Peter Redford, Rodney A Welch
Jun 5, 2013·Frontiers in Biology·Xinghong YangDavid W Pascual
Aug 5, 1998·Research in Veterinary Science·P H ElzerG G Schurig
Dec 12, 2012·Microbial Pathogenesis·Raquel Conde-ÁlvarezIgnacio Moriyón
Sep 17, 2011·Science China. Life Sciences·Zhongpeng ZhaoXiliang Wang
Aug 19, 2016·PLoS Neglected Tropical Diseases·Aseem PandeyPaul de Figueiredo
Jun 16, 2010·Journal of Bioscience and Bioengineering·Yoshitake OrikasaTakuji Ohwada
Dec 12, 2017·Current Tropical Medicine Reports·Angela M Arenas-GamboaThomas A Ficht

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