A built-in mechanism to mitigate the spread of insect-resistance and herbicide-tolerance transgenes into weedy rice populations.

PloS One
Chengyi LiuChaoyang Lin

Abstract

The major challenge of cultivating genetically modified (GM) rice (Oryza sativa) at the commercial scale is to prevent the spread of transgenes from GM cultivated rice to its coexisting weedy rice (O. sativa f. spontanea). The strategic development of GM rice with a built-in control mechanism can mitigate transgene spread in weedy rice populations. An RNAi cassette suppressing the expression of the bentazon detoxifying enzyme CYP81A6 was constructed into the T-DNA which contained two tightly linked transgenes expressing the Bt insecticidal protein Cry1Ab and the glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), respectively. GM rice plants developed from this T-DNA were resistant to lepidopteran pests and tolerant to glyphosate, but sensitive to bentazon. The application of bentazon of 2000 mg/L at the rate of 40 mL/m(2), which is approximately the recommended dose for the field application to control common rice weeds, killed all F(2) plants containing the transgenes generated from the Crop-weed hybrids between a GM rice line (CGH-13) and two weedy rice strains (PI-63 and PI-1401). Weedy rice plants containing transgenes from GM rice through gene flow can be selectively killed by the spray of bentazon wh...Continue Reading

Citations

Dec 20, 2014·GM Crops & Food·Gerhart U Ryffel
Sep 5, 2014·Plant Biotechnology Journal·Luca Lombardo
Oct 28, 2015·Plant Biotechnology Journal·Reginald J MillwoodCharles Neal Stewart
May 14, 2014·Plant Physiology·Jonathan Gressel, Avraham A Levy

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Datasets Mentioned

BETA
EU169459

Methods Mentioned

BETA
transgenic
PCR
electrophoresis

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