A C-terminal segment of the V1R vasopressin receptor is unstructured in the crystal structure of its chimera with the maltose-binding protein

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Nallini Vijayarangan AdikesavanMenachem Shoham

Abstract

The V1 vascular vasopressin receptor (V1R) is a G-protein-coupled receptor (GPCR) involved in the regulation of body-fluid osmolality, blood volume and blood pressure. Signal transduction is mediated by the third intracellular loop of this seven-transmembrane protein as well as by the C-terminal cytoplasmic segment. A chimera of the maltose-binding protein (MBP) and the C-terminal segment of V1R has been cloned, expressed, purified and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 51.10, b = 66.56, c = 115.72 A, beta = 95.99 degrees. The 1.8 A crystal structure reveals the conformation of MBP and part of the linker region of this chimera, with the C-terminal segment being unstructured. This may reflect a conformational plasticity in the C-terminal segment that may be necessary for proper function of V1R.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
Aug 5, 2000·Science·K PalczewskiM Miyano
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·M ThibonnierM Shoham
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y LiuG Zhang
Feb 19, 2003·Journal of Molecular Biology·Jeffrey A ChaoJames R Williamson
Jun 26, 2003·Protein Science : a Publication of the Protein Society·Douglas R SmythBostjan Kobe

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Citations

Dec 13, 2005·Journal of Molecular Biology·Hartmut H NiemannDirk W Heinz
Jun 2, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Sinem Torol, Murat Kasap
Dec 20, 2015·Protein Science : a Publication of the Protein Society·David S Waugh
Mar 7, 2014·Chembiochem : a European Journal of Chemical Biology·Hsiao-Chan WangChun-Cheng Lin

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