A c-type cytochrome and a transcriptional regulator responsible for enhanced extracellular electron transfer in Geobacter sulfurreducens revealed by adaptive evolution

Environmental Microbiology
Pier-Luc TremblayDerek R Lovley

Abstract

The stimulation of subsurface microbial metabolism often associated with engineered bioremediation of groundwater contaminants presents subsurface microorganisms, which are adapted for slow growth and metabolism in the subsurface, with new selective pressures. In order to better understand how Geobacter species might adapt to selective pressure for faster metal reduction in the subsurface, Geobacter sulfurreducens was put under selective pressure for rapid Fe(III) oxide reduction. The genomes of two resultant strains with rates of Fe(III) oxide reduction that were 10-fold higher than those of the parent strain were resequenced. Both strains contain either a single base-pair change or a 1 nucleotide insertion in a GEMM riboswitch upstream of GSU1761, a gene coding for the periplasmic c-type cytochrome designated PgcA. GSU1771, a gene coding for a SARP regulator, was also mutated in both strains. Introduction of either of the GEMM riboswitch mutations upstream of pgcA in the wild-type increased the abundance of pgcA transcripts, consistent with increased expression of pgcA in the adapted strains. One of the mutations doubled the rate of Fe(III) oxide reduction. Interruption of GSU1771 doubled the Fe(III) oxide reduction rate. Thi...Continue Reading

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Citations

Mar 8, 2011·Applied and Environmental Microbiology·Kelly P NevinDerek R Lovley
Jul 3, 2013·Microbial Cell Factories·Martin Dragosits, Diethard Mattanovich
Jul 4, 2012·Annual Review of Microbiology·Derek R Lovley
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Colleen A KellenbergerMing C Hammond
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·James W NelsonRonald R Breaker
Apr 19, 2011·Current Opinion in Biotechnology·Vasiliy A PortnoyKarsten Zengler
Jul 8, 2011·Molecular Systems Biology·Tom M ConradBernhard Ø Palsson
Feb 1, 2012·Environmental Microbiology Reports·Pier-Luc TremblayDerek Lovley
Nov 28, 2012·Biochemical Society Transactions·Derek R Lovley
Feb 18, 2017·Chemical Society Reviews·Cui-E ZhaoJun-Jie Zhu
May 23, 2012·ChemSusChem·Nikhil S Malvankar, Derek R Lovley
May 13, 2014·Applied and Environmental Microbiology·Jessica A SmithDerek R Lovley
May 12, 2012·ChemSusChem·Ashley E FranksDerek R Lovley
Jul 28, 2019·Scientific Reports·Tian ZhangPier-Luc Tremblay
Aug 20, 2017·Biointerphases·Wenchao WeiXiao-Ying Yu
Jul 31, 2018·Frontiers in Microbiology·Toshiyuki UekiDerek R Lovley
Jun 15, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ying LiuDaniel R Bond
Dec 12, 2020·The Science of the Total Environment·Xunan Yang, Shanshan Chen
Mar 21, 2021·Applied and Environmental Microbiology·Toshiyuki Ueki
Jun 3, 2021·Antioxidants·Tomás M FernandesCarlos A Salgueiro
Jul 3, 2020·Analytical Chemistry·Wenchao WeiXiao-Ying Yu

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