PMID: 8581969Nov 1, 1995Paper

A cADP-ribose antagonist does not inhibit secretagogue-, caffeine- and nitric oxide-induced Ca2+ responses in rat pancreatic beta-cells

Cell Calcium
N J WillmottP A Smith

Abstract

It is controversial whether the Ca2+ mobilizing agent, cADP-ribose (cADPR), is implicated in secretagogue-mediated intracellular Ca2+ responses of pancreatic beta-cells. In this study we utilised a potent antagonist of cADPR, 8-amino-cADPR, to determine whether cADPR is involved in glucose-, acetylcholine-, caffeine- and nitric oxide-induced intracellular Ca2+ responses of isolated rat beta-cells. The antagonist was found to be effective in the complete inhibition of cADPR-induced Ca2+ release from sea urchin egg microsome preparations, when used at equivalent concentrations to cADPR (between 0.1-10 microM) in the assay. Isolated beta-cells were co-loaded with up to 50 microM 8-amino-cADPR, and Fura-2 or Fluo-3, by the whole-cell patch technique. At this concentration, the antagonist failed to affect standard glucose- and acetylcholine-induced increases in the intracellular free Ca2+ ([Ca2+]i) of isolated rat pancreatic beta-cells, as assessed by video ratio imaging and single wavelength microfluorimetry. Applying the same methodology, the antagonist also failed to affect NO- and caffeine-induced intracellular Ca2+ responses of rat beta-cells. These results suggest that cADPR does not appear to play a fundamental role in beta-c...Continue Reading

References

Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Pusch, E Neher
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Aug 1, 1994·Cell Calcium·G A RutterC B Wollheim
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Citations

May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M S IslamP O Berggren
Sep 23, 2014·Cell Calcium·Patrick GilonMagalie A Ravier
Nov 26, 2010·Islets·Abdelilah ArredouaniAntony Galione
Dec 12, 2002·Diabetes·Jose M Cancela, Ole H Petersen
Jun 9, 2012·Acta Biochimica Et Biophysica Sinica·Yongjuan ZhaoHon Cheung Lee
Jun 27, 2006·Journal of Cellular and Molecular Medicine·Andrew Y Zhang, Pin-Lan Li
Oct 1, 1996·The American Journal of Physiology·T P DousaK W Beers
Feb 24, 1997·Biochemical and Biophysical Research Communications·W J MalaisseT Katada
Jun 22, 1999·Cellular Signalling·A H Guse

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