A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm

Reproduction : the Official Journal of the Society for the Study of Fertility
M Rocio Servin-VencesTakuya Nishigaki

Abstract

Progesterone is a physiological agonist for mammalian sperm, modulating its flagellar movement and facilitating the acrosome reaction. To study the initial action of progesterone, we developed a caged analog with a photosensitive group: nitrophenylethanediol, at position 20. Using this compound combined with stroboscopic illumination, we performed Ca(2)(+) imaging of human spermatozoa and analyzed the effects of progesterone on the intracellular Ca(2)(+) concentration ([Ca(2)(+)](i)) of beating flagella for the first time. We observed a transient [Ca(2)(+)](i) increase in the head and the flagellum upon photolysis of the caged progesterone and an increase in flagellar curvature. Detailed kinetic analysis revealed that progesterone elicits an increase in the [Ca(2)(+)](i) immediately in the flagellum (mid-piece and principal piece), thereafter in the head with a short time lag. This observation is different from the progesterone-induced Ca(2)(+) mobilization in mouse spermatozoa, where the Ca(2)(+) rise initiates at the base of the sperm head. Our finding is mostly consistent with the recent discovery that progesterone activates CatSper channels in human spermatozoa, but not in mouse spermatozoa.

References

Apr 28, 1989·Biochemical and Biophysical Research Communications·R A OsmanS Meizel
May 18, 1999·Biology of Reproduction·B S JaiswalM Eisenbach
Jun 6, 2000·Developmental Biology·J C Kirkman-BrownS J Publicover
Jun 8, 2001·Biochemical and Biophysical Research Communications·T NishigakiA Darszon
Jun 28, 2001·Physiological Reviews·A Aranda, A Pascual
Feb 4, 2003·Nature Cell Biology·U Benjamin KauppIngo Weyand
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhuPeter Thomas
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhuPeter Thomas
Mar 5, 2003·The Biochemical Journal·Claire V HarperStephen J Publicover
Apr 16, 2003·The Journal of Cell Biology·Kiyoko FukamiTadaomi Takenawa
Jul 5, 2003·Physiological Reviews·Ralf M LoselMartin Wehling
Nov 11, 2003·The Biochemical Journal·Jackson C Kirkman-BrownStephen J Publicover
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anne E CarlsonDonner F Babcock
Jun 2, 2005·The Journal of Cell Biology·Christopher D WoodAlberto Darszon
Jul 8, 2005·The EMBO Journal·Martin BöhmerUlrich Benjamin Kaupp
May 5, 2007·The Journal of Biological Chemistry·Jin LiuDejian Ren
Jun 8, 2007·Biology of Reproduction·Jingsheng XiaDejian Ren
Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kogiku ShibaManabu Yoshida
Mar 5, 2009·Journal of the American Chemical Society·Funda KilicU Benjamin Kaupp
Nov 26, 2009·Human Reproduction Update·Trevor G CooperKirsten M Vogelsong
May 4, 2010·Developmental Biology·Adan GuerreroAlberto Darszon
Jan 13, 2011·Nature Communications·Jean-Ju ChungDavid E Clapham
Mar 18, 2011·Nature·Timo StrünkerU Benjamin Kaupp
Mar 18, 2011·Nature·Polina V LishkoYuriy Kirichok
May 19, 2011·Science Signaling·Dejian Ren

❮ Previous
Next ❯

Citations

Jan 25, 2013·The Journal of Biological Chemistry·Wardah AlasmariChristopher L R Barratt
Feb 15, 2013·Human Reproduction·Wardah AlasmariSenga K Oxenham
Mar 7, 2014·Pflügers Archiv : European journal of physiology·Alberto Darszon, Arturo Hernández-Cruz
Dec 12, 2012·Bioscience, Biotechnology, and Biochemistry·Ayako KikuchiOsamu Numata
Oct 3, 2013·Protoplasma·Hitoshi Sugiyama, Douglas E Chandler
Apr 10, 2014·Biochimica Et Biophysica Acta·Doris HöglingerCarsten Schultz
Mar 29, 2014·ELife·Christoph BrenkerTimo Strünker
Sep 13, 2012·International Journal of Andrology·Martin Blomberg Jensen, Stephen J Publicover
Mar 24, 2016·Scientific Reports·Zhuoran ZhangYu Sun
Mar 24, 2016·ELife·Shatanik MukherjeeDagmar Wachten
Sep 5, 2012·The Biochemical Journal·Linda LefièvreStephen Publicover
Nov 9, 2016·Trends in Cell Biology·U B Kaupp, T Strünker
Aug 8, 2014·Biology of Reproduction·Claudia Sánchez-CárdenasAlberto Darszon
Jan 8, 2017·Cold Spring Harbor Perspectives in Biology·Dagmar WachtenU Benjamin Kaupp
Apr 14, 2016·Reproductive Sciences·Aideé Saray López-Torres, Mayel Chirinos
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva
Dec 22, 2019·Scientific Reports·Sergio A MachadoDavid J Miller
Feb 1, 2017·Reproductive Sciences·Aideé Saray López-Torres, Mayel Chirinos
Aug 13, 2021·Frontiers in Cell and Developmental Biology·Janice K JeschkeChristoph Brenker
Aug 29, 2021·Bio Systems·Esperanza Mata-MartínezAlberto Darszon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.