A Cajal body-independent pathway for telomerase trafficking in mice.

Experimental Cell Research
Rebecca L TomlinsonMichael P Terns

Abstract

The intranuclear trafficking of human telomerase involves a dynamic interplay between multiple nuclear sites, most notably Cajal bodies and telomeres. Cajal bodies are proposed to serve as sites of telomerase maturation, storage, and assembly, as well as to function in the cell cycle-regulated delivery of telomerase to telomeres in human cells. Here, we find that telomerase RNA does not localize to Cajal bodies in mouse cells, and instead resides in separate nuclear foci throughout much of the cell cycle. However, as in humans, mouse telomerase RNA (mTR) localizes to subsets of telomeres specifically during S phase. The localization of mTR to telomeres in mouse cells does not require coilin-containing Cajal bodies, as mTR is found at telomeres at similar frequencies in cells from wild-type and coilin knockout mice. At the same time, we find that human TR localizes to Cajal bodies (as well as telomeres) in mouse cells, indicating that the distinct trafficking of mTR is attributable to an intrinsic property of the RNA (rather than a difference in the mouse cell environment such as the properties of mouse Cajal bodies). We also find that during S phase, mTR foci coalesce into short chains, with at least one of the conjoined mTR fo...Continue Reading

References

Sep 27, 1990·Nature·D Kipling, H J Cooke
Dec 1, 1990·Molecular and Cellular Biology·K G Ten HagenS N Cohen
Dec 1, 1994·The Journal of Cell Biology·U T Meier, G Blobel
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K R ProwseC W Greider
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J W Shay, S Bacchetti
Jul 31, 1998·American Journal of Human Genetics·A G Matera, M R Frey
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L Martín-RiveraM A Blasco
Sep 2, 1999·Experimental Cell Research·W E WrightJ W Shay
Mar 18, 2000·Cell·J L ChenC W Greider
Feb 19, 2002·Oncogene·Kathleen Collins, James R Mitchell
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yie LiuLea Harrington
Apr 17, 2002·The Journal of Biological Chemistry·Katherine T EtheridgeChristopher M Counter
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Steven E ArtandiRonald A DePinho
Jun 27, 2002·Experimental Cell Research·Yinhua YangSherman M Weissman
Aug 29, 2002·Nature Cell Biology·Judy M Y WongKathleen Collins
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Yu-Sheng CongJerry W Shay
Oct 4, 2002·Developmental Cell·Michael D HebertA Gregory Matera
Oct 16, 2002·Nature Genetics·Marta García-CaoMaría A Blasco
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Joseph G Gall
Feb 26, 2004·The Journal of Cell Biology·Beáta E JádyTamás Kiss
Jun 2, 2005·Mutation Research·Sandy Chang
Sep 17, 2005·Genes & Development·Titia de Lange
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Mario Cioce, Angus I Lamond
Sep 23, 2006·The New England Journal of Medicine·Steven E Artandi
Sep 19, 2007·Nature Chemical Biology·Maria A Blasco
Feb 8, 2008·Nature Reviews. Cancer·Calvin B Harley
May 27, 2008·Nature Reviews. Cancer·Yibin DengSandy Chang
Aug 6, 2008·Annual Review of Genetics·Wilhelm Palm, Titia de Lange

❮ Previous
Next ❯

Citations

Jun 26, 2012·Trends in Biochemical Sciences·J Arturo Londoño-Vallejo, Raymund J Wellinger
Oct 9, 2012·Wiley Interdisciplinary Reviews. RNA·Martin MachynaKarla M Neugebauer
May 15, 2015·RNA Biology·Martin MachynaDavid Staněk
May 1, 2012·Current Opinion in Cell Biology·Miroslav Dundr
Nov 19, 2011·Mutation Research·Joshua D Podlevsky, Julian J-L Chen
Apr 28, 2016·Pharmacology & Therapeutics·Yinnan Chen, Yanmin Zhang
Sep 21, 2016·Genes·Deanna Elise MacNeilChantal Autexier
Jul 14, 2010·Biochemical and Biophysical Research Communications·Guoyuan ChenMujun Zhao
Jan 25, 2017·Journal of Molecular Biology·Yulia Vasianovich, Raymund J Wellinger
May 2, 2012·Molecular and Cellular Biology·J Lewis SternTracy M Bryan
Nov 20, 2015·Nature Structural & Molecular Biology·Dirk Hockemeyer, Kathleen Collins
Sep 18, 2013·International Journal of Oncology·Diego L Mengual GómezDaniel E Gómez
Jul 15, 2015·The Journal of Biological Chemistry·Jacob M Vogan, Kathleen Collins
Apr 4, 2020·Nature Reviews. Molecular Cell Biology·Caitlin M Roake, Steven E Artandi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.