A calcium-modulated plasmonic switch.

Journal of the American Chemical Society
W Paige HallRichard P Van Duyne

Abstract

A plasmonic switch based on the calcium-induced conformational changes of calmodulin is shown to exhibit reversible wavelength modulations in response to changing calcium concentration. The extinction maximum (lambdamax) of a localized surface plasmon resonance (LSPR) sensor functionalized with a novel calmodulin construct, cutinase-calmodulin-cutinase (CutCaMCut), reversibly shifts by 2-3 nm. A high-resolution (HR) LSPR spectrometer with a wavelength resolution (3sigma) of 1.5 x 10-2 nm was developed to detect these wavelength modulations in real-time, providing information about the dynamics and structure of the protein. The rate of conversion from open (Ca2+-bound) to closed (Ca2+-free) calmodulin is shown to be 4-fold faster than the reverse process, with a closing rate of 0.127 s-1 and opening rate of 0.034 s-1. As far as we are aware, this plasmonic switch marks the first use of LSPR spectroscopy to detect reversible conformational changes in an unlabeled protein.

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Citations

Dec 14, 2011·Analytical Chemistry·Francis P ZamboriniRadhika Dasari
Mar 13, 2012·Analytical Chemistry·Daniele Dell'OrcoKarl-Wilhelm Koch
Jun 9, 2011·Chemical Reviews·Matthew R JonesChad A Mirkin
Nov 26, 2010·Journal of the American Chemical Society·Julia M BinghamRichard P Van Duyne
Feb 2, 2011·Nano Letters·W Paige HallRichard P Van Duyne
May 10, 2013·Nanoscale·Khaldoun Abdul-KaderAzzedine Bousseksou
Oct 27, 2011·Nanomedicine·Laura B SagleRichard P Van Duyne
Sep 1, 2008·Biointerphases·Magnus P JonssonFredrik Höök
Apr 11, 2014·Chemical Communications : Chem Comm·Yun ZhaoXing-Hua Xia
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Oct 25, 2011·Biochimica Et Biophysica Acta·Karl-Wilhelm Koch
Jul 6, 2016·ACS Chemical Biology·Daniele Dell'Orco, Karl-Wilhelm Koch
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Apr 22, 2014·Chemical Reviews·Onur TokelUtkan Demirci
Jun 11, 2011·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·W Paige HallRichard P Van Duyne

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