A Calibration Curve Implanted Enzyme-Linked Immunosorbent Assay for Simultaneously Quantitative Determination of Multiplex Mycotoxins in Cereal Samples, Soybean and Peanut.

Toxins
Yuxiang WuZhiqiang Shen

Abstract

In this study, a rapid and sensitive immunoassay method has been established based on calibration curve implanted enzyme-linked immunosorbent assay (C-ELISA) for the simultaneously quantitative determination of aflatoxin B1, deoxynivalenol and zearalenone in cereal samples, soybean and peanut. The C-ELISA avoids using the standard substances during the detection. The principle of the C-ELISA is to implant the optimized standard curve data into the matched analysis software which can make data processing more convenient and faster. The implanted calibration curve software was programmed with C plus plus. In the new immunoassay system for aflatoxin B1, deoxynivalenol and zearalenone, their linear detection ranges were from 0.03~0.81, 1.00~27.00 and 5.00~135.00 ng/g, respectively. Recovery rates from spiked samples ranged from 85% to 110% with the intra-assay coefficients of variation under 5%. Compared with HPLC method, the new method showed consistence in all the observed contents of the three mycotoxins in real samples. The new method can rapidly and reliably high throughput simultaneously screen for multiplex mycotoxins.

References

Feb 16, 2002·International Journal of Food Microbiology·J OlssonJ Schnürer
Dec 27, 2008·Analytica Chimica Acta·Nicholas W TurnerSergey A Piletsky
Feb 22, 2012·Biosensors & Bioelectronics·Ying WangZhixian Gao
Dec 22, 2012·Journal of Agricultural and Food Chemistry·Fadwa Al-TaherJack Cappozzo
Apr 22, 2014·Analytical Chemistry·Suquan SongAibo Wu
Jan 31, 2015·Toxins·Alexandr E UrusovBoris B Dzantiev
Mar 8, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhaowei ZhangPeiwu Li
Mar 29, 2016·Biosensors & Bioelectronics·Ruchika ChauhanB D Malhotra
Dec 13, 2016·Journal of Agricultural and Food Chemistry·Astrid FoubertSarah De Saeger
Jan 25, 2020·Journal of Agricultural and Food Chemistry·Jia-Xiang YanQing-Hua He

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
C-ELISA
Assay
ELISA

Software Mentioned

Origin9
Origin
Windows XP

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