A calorimetric and infrared spectroscopic study of the stabilizing solute proline

Biophysical Journal
A S Rudolph, J H Crowe

Abstract

We have studied the calorimetric and infrared spectroscopic properties of the amino acid proline which has been implicated in the stabilization of biomacromolecules during reduced water states. It has been suggested that the ability of this molecule to protect biomacromolecules during these stress states may be related to the formation of polymeric aggregates of proline monomers in solution. The structure of this aggregate is thought to be an alternates stack, forming a hydrophilic colloid-like polymer which is thought to interact with hydrophobic moieties of biomacromolecules, reducing the exposed hydrophobic area during reduced water conditions. Calorimetric data presented in this work show that in increasing concentration of proline in solution the enthalpy associated with the melting of bulk water is greatly reduced, indicating strong hydrogen bonding character of proline in aqueous solution. Proline shows two eutectic phase separations at moderate concentrations and one of these eutectics may be the proposed intermolecular state. A partial phase diagram for proline is presented. Fourier-transform infrared spectroscopic data indicate that the COO- asymmetric stretch of proline shows marked splitting with increasing proline ...Continue Reading

Citations

Jul 19, 2011·Advanced Drug Delivery Reviews·Diwakar ShuklaBernhardt L Trout
Jun 28, 2015·International Journal of Biological Macromolecules·Vera A BorzovaBoris Kurganov
Jul 28, 2017·Physical Chemistry Chemical Physics : PCCP·Olga A DmitrievaRichard Buchner
Mar 3, 2018·The Journal of Experimental Biology·Jan RozsypalVladimír Koštál
May 12, 2018·Biological Reviews of the Cambridge Philosophical Society·Jantina Toxopeus, Brent J Sinclair
Aug 5, 2016·The Journal of Experimental Biology·Vladimír KoštálPetr Šimek
Oct 24, 2019·Proceedings. Biological Sciences·Lauren E Des MarteauxVladimír Koštál
Oct 7, 2017·Physical Chemistry Chemical Physics : PCCP·Paula Malo de MolinaJuan Colmenero
May 23, 2018·Food Chemistry·Monica PrimacellaTong Wang
Apr 5, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Astrid HauptmannThomas Loerting
Nov 11, 2014·The Journal of Physical Chemistry. B·Sebastian BuschSylvia E McLain
May 29, 2019·The Journal of Physical Chemistry. B·Rachel M AbaskharonFeng Gai
Jun 27, 2007·The Journal of Physical Chemistry. B·R Z TroitzschJ Crain
Dec 30, 1988·Journal of Chromatography·T Cserháti, M Szögyi

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