PMID: 10020Sep 1, 1976

A calorimetric study of polyguanylic acid at neutral pH

Biophysical Chemistry
H Klump

Abstract

The structure of polyguanylic acid (poly G) at neutral pH has been studied by optical and calorimetrical methods. It can be shown that diverging from earlier findings Poly G reversibly undergoes a cooperative thermal transition. Thermal denaturation curves are recorded at 253 nm as a function of the sodium ion concentration. The denaturation enthalpy of poly G in dilute aqueous solution is determined to 2.2 kcal/mole g. It is concluded, that the part of the ordered poly G structure, which gives rise to a temperature dependent cooperative transition, arises from stacking interactions of adjacent bases in the single strand.

References

Sep 6, 1965·Biochimica Et Biophysica Acta·M N ThangM Grunberg-Manago

Related Concepts

Calorimetry
Hydrogen-Ion Concentration
RNA Conformation
Poly G
Polyribonucleotides
Thermodynamics

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