PMID: 7035837Jan 1, 1981Paper

A carbon catabolite repression mutant of Saccharomyces cerevisiae with elevated hexokinase activity: evidence for regulatory control of hexokinase PII synthesis

Molecular & General Genetics : MGG
K D Entian

Abstract

Mutants were investigated that had elevated hexokinase activity and had been isolated previously as resistant to carbon catabolite repression (Zimmermann and Scheel 1977). They were allele tested with mutant strains of Lobo and Maitra (1977), which had defects in one or more of the genes coding for glucokinase and unspecific hexokinases. It was shown, that the mutation abolishing carbon catabolite repression had occurred in a gene that was not allelic to any of the structural genes coding for hexokinases. This indicated that a regulatory defect was responsible for elevated hexokinase activity. This agreed with observations that hexokinase activities were like wild-type during growth on non-fermentable carbon sources in hex2 mutants. Recombination between the mutant allele hex2 and mutant alleles hxk1 and hxk2, coding for hexokinase PI and PII respectively, clearly demonstrated that only hexokinase PII was elevated in hex2 mutants. When hex2 mutant cells grown on YEP ethanol were shifted to YEP glucose media, hexokinase activity increased after 30 min. This increase depended on de novo protein synthesis. hex2 mutants provide evidence, that carbon catabolite repression and synthesis of hexokinase PII are under common regulatory c...Continue Reading

References

Feb 28, 1977·Molecular & General Genetics : MGG·F K ZimmermannP Haubetamann
Jul 7, 1977·Molecular & General Genetics : MGG·F K Zimmermann, I Scheel
Sep 1, 1979·Molecular & General Genetics : MGG·M K Grossmann, F K Zimmermann
Jan 1, 1974·Molecular & General Genetics : MGG·F K Zimmermann, N R Eaton

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Citations

Sep 1, 1991·European Journal of Biochemistry·D Niederacher, K D Entian
Mar 1, 1987·Molecular & General Genetics : MGG·D Niederacher, K D Entian
Jan 1, 1986·CRC Critical Reviews in Biochemistry·S D HanesK A Bostian
Jun 19, 2008·FEMS Microbiology Reviews·Juana M Gancedo
Jan 1, 1984·Molecular & General Genetics : MGG·K U FröhlichD Mecke
Jan 1, 1983·Biotechnology Advances·R Hütter, P Niederberger

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