A carbon free filter for collection of large volume samples of cellular biomass from oligotrophic waters.

Journal of Microbiological Methods
Brian J MaillouxGreg F Slater

Abstract

Isotopic analysis of cellular biomass has greatly improved our understanding of carbon cycling in the environment. Compound specific radiocarbon analysis (CSRA) of cellular biomass is being increasingly applied in a number of fields. However, it is often difficult to collect sufficient cellular biomass for analysis from oligotrophic waters because easy-to-use filtering methods that are free of carbon contaminants do not exist. The goal of this work was to develop a new column based filter to autonomously collect high volume samples of biomass from oligotrophic waters for CSRA using material that can be baked at 450°C to remove potential organic contaminants. A series of filter materials were tested, including uncoated sand, ferrihydrite-coated sand, goethite-coated sand, aluminum-coated sand, uncoated glass wool, ferrihydrite-coated glass wool, and aluminum-coated glass wool, in the lab with 0.1 and 1.0 μm microspheres and Escherichia coli. Results indicated that aluminum-coated glass wool was the most efficient filter and that the retention capacity of the filter far exceeded the biomass requirements for CSRA. Results from laboratory tests indicate that for oligotrophic waters with 1×10(5) cells ml(-1), 117l of water would nee...Continue Reading

References

Jan 1, 1994·Annual Review of Microbiology·K H Nealson, D Saffarini
Apr 7, 2004·Science·J Craig VenterHamilton O Smith
May 12, 2005·Environmental Science & Technology·Gregory F SlaterChristopher M Reddy
Mar 25, 2008·Applied and Environmental Microbiology·Elisabetta LambertiniMark A Borchardt
May 26, 2010·Ground Water·Peter S K KnappettAlexander van Geen
Jun 4, 2011·Nature·G BorgonieT C Onstott

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