A carbon nanofiber-based label free immunosensor for high sensitive detection of recombinant bovine somatotropin

Biosensors & Bioelectronics
Syazana Abdullah Lim, Minhaz Uddin Ahmed

Abstract

A carbon nanofiber-based label free electrochemical immunosensor for sensitive detection of recombinant bovine somatotropin (rbST) was developed. In this immunosensor design, a mild site-directed antibody immobilization via interaction of boronic acid and oligosaccharide moiety found on Fc region of an antibody was performed to preserve the biological activity of antibody and improve the sensor's sensitivity. Electrochemical characterization of the immunosensor fabrication was carried out by differential pulse voltammetry (DPV) in Fe(CN)6(3-)/Fe(CN)6(4-) probe. A comparison study between different transducer platforms showed carbon nanofiber gave higher current signal response than single-walled carbon nanotube. In this work, calibration curve was obtained from the decrease of DPV peak current of Fe(CN)6(3-)/Fe(CN)6(4-) after immunocomplexed was formed. A linear relationship between DPV current change signal response and rbST concentrations from 1pg/mL to 10ng/mL (correlation coefficient of 0.9721) was achieved with detection limit of 1pg/mL. Our developed immunosensor demonstrated high selectivity in cross-reactivity studies and a good percentage recovery in spiked bovine serum sample.

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Citations

Jul 5, 2016·Frontiers in Neuroscience·Michele BaldrighiSilvia Giordani
Dec 10, 2015·Analytical and Bioanalytical Chemistry·Xianwen MaoGregory C Rutledge
Jun 16, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Syazana A Lim, Minhaz U Ahmed
Jan 10, 2018·Sensors·Mohammad RizwanMinhaz Uddin Ahmed
Jan 20, 2021·Analytica Chimica Acta·Thangapandi KalyaniSaikat Kumar Jana
Mar 23, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ghada G AbdoAhmed A Elzatahry

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