A carbon sink pathway increases carbon productivity in cyanobacteria

Metabolic Engineering
John W K Oliver, Shota Atsumi

Abstract

The burning of fossil reserves, and subsequent release of carbon into the atmosphere is depleting the supply of carbon-based molecules used for synthetic materials including plastics, oils, medicines, and glues. To provide for future society, innovations are needed for the conversion of waste carbon (CO2) into organic carbon useful for materials. Chemical production directly from photosynthesis is a nascent technology, with great promise for capture of CO2 using sunlight. To improve low yields, it has been proposed that photosynthetic capacity can be increased by a relaxation of bottlenecks inherent to growth. The limits of carbon partitioning away from growth within the cell and the effect of partitioning on carbon fixation are not well known. Here we show that expressing genes in a pathway between carbon fixation and pyruvate increases partitioning to 2,3-butanediol (23BD) and leads to a 1.8-fold increase in total carbon yield in the cyanobacterium Synechococcus elongatus PCC 7942. Specific 2,3-butanediol production increases 2.4-fold. As partitioning increases beyond 30%, it leads to a steep decline in total carbon yield. The data suggests a local maximum for carbon partitioning from the Calvin Benson cycle that is scalable ...Continue Reading

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Citations

May 6, 2015·Frontiers in Bioengineering and Biotechnology·Henning Knoop, Ralf Steuer
May 31, 2016·Journal of Biotechnology·Anna E Case, Shota Atsumi
Sep 11, 2016·Current Opinion in Chemical Biology·Neal J OliverShota Atsumi
Oct 16, 2016·Plant & Cell Physiology·Bradley W AbramsonDaniel C Ducat
Oct 4, 2017·The Journal of Biological Chemistry·Cory J KnootHimadri B Pakrasi
Mar 14, 2017·Nature Communications·Masahiro KannoShota Atsumi
Mar 23, 2017·Biodegradation·Shiping ZhangYiu Fai Tsang
Dec 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xin WangJoshua S Yuan
Sep 7, 2017·FEMS Microbiology Letters·Angela ZhangShota Atsumi
Nov 25, 2020·Trends in Microbiology·Amit SrivastavaPratyoosh Shukla
Feb 28, 2021·Cell·Yuzhong LiuJay D Keasling
May 10, 2017·Metabolic Engineering·Lara J JazminJamey D Young
Apr 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·María Santos-MerinoDaniel C Ducat
Aug 20, 2021·Current Opinion in Biotechnology·Xufeng LiuPeter Lindblad
Oct 16, 2021·Journal of Agricultural and Food Chemistry·Haojie JinYujie Fu

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