A carboxyl-terminal domain in fibroblast growth factor (FGF)-2 inhibits FGF-1 release in response to heat shock in vitro.

The Journal of Biological Chemistry
J ShiT Maciag

Abstract

The fibroblast growth factor (FGF) prototypes, FGF-1 and FGF-2, lack a signal sequence, but both contain a nuclear localization sequence. We prepared a series of FGF-1 deletion mutants fused to the reporter gene, beta-galactosidase (beta-gal) and determined that a domain between residues 83 and 154 is responsible for FGF-1 cytosol retention in NIH 3T3 cells. Using a series of FGF-beta-gal chimeric proteins prepared by the shuffling of cassette-formatted synthetic FGF prototype genes, we were able to demonstrate that the nuclear localization sequence from the 5'-CUG region of FGF-2 is not able to direct the nuclear association of FGF-1 due to its inability to repress the function of the FGF-1 cytosol retention domain. We also observed that while the FGF-1:beta-gal chimera was released in response to heat shock, the FGF-2:beta-gal protein was not. Further, replacement of the FGF-1 cytosol retention domain with the corresponding domain from FGF-2 repressed the release of the chimeric protein. These data suggest that the specificity of the stress-induced secretion pathway for FGF-1 involves a carboxyl-terminal domain that is absent in FGF-2 and that the FGF-1 secretion pathway does not restrict the release of high molecular weight ...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A JacksonT Maciag
Nov 16, 1992·Biochemical and Biophysical Research Communications·X ZhanT Maciag
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jan 1, 1989·Annual Review of Biochemistry·W H Burgess, T Maciag
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R Z Florkiewicz, A Sommer
Jan 1, 1989·Molecular and Cellular Biology·D Martin-ZancaM Barbacid
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R E Friesel, T Maciag
Jan 1, 1993·Annual Review of Biochemistry·W J FantlL T Williams

❮ Previous
Next ❯

Citations

Oct 8, 1999·The Biochemical Journal·E MizukoshiT Imamura
Jun 21, 2001·Biochemical and Biophysical Research Communications·Y D SohnY Jang
Feb 24, 2009·Biochemical and Biophysical Research Communications·Irene GrazianiIgor Prudovsky
Mar 17, 2009·Biochemical and Biophysical Research Communications·Sepuru K MohanChin Yu
Jan 28, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·D G NeschisM A Golden
Aug 25, 2001·Growth Factors·C Mouta CarreiraT Maciag
Aug 26, 1998·The Journal of Biological Chemistry·T M LaValleeT Maciag
Aug 26, 1998·The Journal of Biological Chemistry·C Mouta CarreiraT Maciag
Nov 23, 2000·The Journal of Biological Chemistry·F TarantiniT Maciag
Nov 20, 2003·Journal of Cell Science·Igor PrudovskyThomas Maciag

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.