A cartilage-derived growth factor enhances hyaluronate synthesis and diminishes sulfated glycosaminoglycan synthesis in chondrocytes

Journal of Cellular Physiology
D HamermanM Klagsbrun

Abstract

Cartilage-derived growth factor purified to homogeneity by affinity chromatography on columns of heparin-Sepharose was mitogenic for early passage bovine fetal chondrocytes. Hyaluronate and sulfated glycosaminoglycan synthesis in these cells was analyzed by differential enzymatic digestion of the glycosaminoglycans labeled with [14C] glucosamine or [35S]. It was found that chondrocyte proliferation was accompanied by about a four-fold increase in hyaluronate synthesis over a two-day period, while the synthesis of sulfated glycosaminoglycans decreased by about 2-fold. Chromatographic analysis of the sulfated glycosaminoglycans showed decreases in chondroitin 4 and 6 sulfates. It was concluded from these results that cartilage-derived growth factor was a proliferative factor for chondrocytes and differed from the somatomedins.

References

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Citations

Nov 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W O TwalR M Leach
Jul 1, 1996·In Vitro Cellular & Developmental Biology. Animal·W O Twal, R M Leach
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S I MunaimB P Toole
Apr 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J HitzemanH L Hosick
Apr 3, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shuhei OtsukiMitsuo Kinoshita
Sep 1, 1988·Differentiation; Research in Biological Diversity·A L Mescher, C A Cox
Apr 10, 2016·Developmental Biology·Neil McCarthyJohann K Eberhart
Mar 1, 1990·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D G Jones, R L Smith
Sep 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S JingushiM E Bolander
Nov 1, 1990·American Journal of Medical Genetics·R StanescuP Maroteaux
Dec 1, 1990·Journal of the American Geriatrics Society·R L PierronT Stewart
Feb 1, 1993·The Journal of Bone and Joint Surgery. American Volume·S B TrippelS R Doctrow
May 18, 1989·The New England Journal of Medicine·D Hamerman

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