A case report demonstrating the utility of next generation sequencing in analyzing serial samples from the lung following an infection with influenza A (H7N9) virus

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Yongfeng HuQ Jin

Abstract

Bacterial pneumonia is a well-recognized sequela of patient suffering from influenza, and a key factor, with cytokine dysregulation, that contribute to severe disease and mortality. To obtain a comprehensive assessment of lung microbial community dynamics in a fatal influenza H7N9 case during the whole clinical course, we undertook a longitudinal study. Serial bronchoalveolar lavage fluid samples were collected from a H7N9 patient after illness onset, and the microbiome was characterized by using next-generation sequencing and microbiological approaches. Furthermore, the kinetics of circulating cytokine storms related to viral and secondary bacterial infection were analyzed. Within complex and dynamic communities, the lung microbiome with H7N9 infection were dominated by gram-negative bacteria, Acinetobacter baumannii after the viral invasion and during the whole clinical course. Sputum and blood culture confirmed the secondary bacterial infection with multidrug-resistant A. baumannii 9 days later. The dynamics of the bacterial infection with carbapenem-resistant A. baumannii correlated with antibiotic therapy. Our observations also indicated that sustained high levels of host inflammatory factors, consisting of a set of distin...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 27, 2007·Genome Research·Daniel H HusonStephan C Schuster
Feb 15, 2008·The New England Journal of Medicine·Joseph P Mizgerd
May 30, 2008·The New England Journal of Medicine·Michael Eisenhut
Apr 6, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrea EndimianiRobert A Bonomo
Jan 17, 2013·JAMA : the Journal of the American Medical Association·Daniel S Chertow, Matthew J Memoli
Aug 31, 2013·The Journal of Infectious Diseases·Ying ChiMinghao Zhou
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhongfang WangJianqing Xu
Jan 31, 2014·Journal of Clinical Microbiology·Yan Wei LimDouglas Conrad
Feb 7, 2014·The New England Journal of Medicine·Richard G Wunderink, Grant W Waterer
Feb 15, 2014·World Journal of Clinical Cases·Federico GobbiZeno Bisoffi
Feb 15, 2014·World Journal of Clinical Cases·Elena LionettiMario La Rosa
Feb 15, 2014·World Journal of Clinical Cases·Raj GopalVikram Kate
Jun 5, 2014·The New England Journal of Medicine·Michael R WilsonCharles Y Chiu
Apr 18, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y HuQ Jin

❮ Previous
Next ❯

Citations

Sep 22, 2017·PLoS Genetics·Miguel ArenasAntonio Amorim
Jul 11, 2018·Frontiers in Immunology·Meredith J CraneAmanda M Jamieson
Dec 10, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ying LiZhao-Hui Tong
Apr 29, 2021·Expert Review of Respiratory Medicine·Tary YinRichard G Douglas
Oct 22, 2021·Frontiers in Cellular and Infection Microbiology·Chunrong HuangYun Feng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Y HuQ Jin
Proceedings of the National Academy of Sciences of the United States of America
Christopher D SibleyMichael G Surette
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
Cheng-jun BanBing Sun
Proceedings of the National Academy of Sciences of the United States of America
Zhongfang WangJianqing Xu
© 2021 Meta ULC. All rights reserved