A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived from OVCAR-8 human ovarian carcinoma cells

Cancer Letters
Mordechai Liscovitch, Dana Ravid

Abstract

Multidrug-resistant MCF-7 breast adenocarcinoma cells (originally named MCF-7/AdrR cells and later re-designated NCI/ADR-RES) have served as an important and widely used research tool during the last two decades. However, the real identity of these cells has been in doubt since 1998 and has since been debated. The origin of NCI/ADR-RES cells has now been revealed by SNP and karyotypic analyses, carried out at the Sanger Institute and the NCI, respectively. The results of these analyses, recently posted on the Web, show that NCI/ADR-RES cells are derived from OVCAR-8 ovarian adenocarcinoma cells. The case of NCI/ADR-RES cells highlights a wide-spread problem of cell line cross-contamination and misidentification. Fortunately, this is a tractable problem that can be avoided by scrupulous genotyping of cell stocks and adoption of a few simple rules in cell culture practice.

References

Jun 5, 1991·Journal of the National Cancer Institute·A MonksA Vaigro-Wolff
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H CowanC E Myers
May 23, 1998·The Journal of Biological Chemistry·R U JänickeA G Porter
Jun 13, 1998·Journal of the National Cancer Institute·D A ScudieroE A Sausville
Oct 3, 1999·International Journal of Cancer. Journal International Du Cancer·R A MacLeodH G Drexler
Mar 4, 2000·Nature Genetics·U ScherfJ N Weinstein
Sep 21, 2000·Journal of the National Cancer Institute·F PirniaM M Borner
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R MastersP G Debenham
Nov 7, 2002·Journal of the National Cancer Institute·Kapil MehtaSen Pathak
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Satoshi NishizukaJohn N Weinstein
Jun 9, 2004·Cancer Letters·Yetrib HathoutCatherine Fenselau
Feb 1, 2005·Clinical & Experimental Metastasis·Erik W ThompsonRobert Clarke

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Citations

May 25, 2007·Cell Biology and Toxicology·Roland M Nardone
Oct 12, 2010·In Vitro Cellular & Developmental Biology. Animal·Raymond W NimsEugene Elmore
Feb 26, 2013·Archives of Pharmacal Research·Seok-Jeong OhMoo-Yeol Lee
Oct 12, 2010·Journal of the National Cancer Institute·Anna Maria CalcagnoSuresh V Ambudkar
Feb 29, 2008·Nucleic Acids Research·Francesca DemichelisMark A Rubin
Jun 21, 2008·Acta Pharmacologica Sinica·Armando RojasHéctor Figueroa
Jul 9, 2008·Biochemistry. Biokhimii︠a︡·A A Stavrovskaya, T P Stromskaya
May 19, 2011·BMC Molecular Biology·Dawn M StultsAndrew J Pierce
Dec 5, 2012·PloS One·Santosh K PatnaikSai Yendamuri
Mar 12, 2011·Regenerative Medicine·Liz Kerrigan, Raymond W Nims
Feb 18, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Aimo Kannt, Thomas Wieland
Jul 6, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Fu QiangHyo-Kyung Han
Feb 24, 2010·Cancer Letters·Victoria C HodgkinsonLynn Cawkwell
Jun 4, 2014·Journal of Natural Products·Lin DuRobert H Cichewicz
Sep 18, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ayman KhdairJayanth Panyam
May 21, 2008·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Bryan P Toole, Mark G Slomiany
Jun 16, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Marc-Olivier BaradezDamian Marshall
Aug 1, 2009·Proteomics. Clinical Applications·Hermann Lage
May 24, 2012·Biotechnology Progress·Annalaura ManciaAnne L Plant
Jan 24, 2013·Proteomics. Clinical Applications·Kenneth D ColeLili Wang
May 4, 2011·Journal of Surgical Oncology·Xiaofang ZhangGengyin Zhou
Oct 28, 2009·International Journal of Cancer. Journal International Du Cancer·Wilhelm G DirksHiroshi Mizusawa
Apr 5, 2014·Human Mutation·Bernard LeroyThierry Soussi
Sep 18, 2009·Head & Neck·J Chad BrennerThomas E Carey
Aug 28, 2009·Journal of Cellular Physiology·Claudia Alexandra DumitruErich Gulbins
May 29, 2015·Nature Methods·Leonard P FreedmanYvonne A Reid
Feb 15, 2012·International Journal of Breast Cancer·N S Wind, I Holen
Aug 13, 2013·Cell Reports·Amin Moghaddas GholamiBernhard Kuster

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