A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.

Nature
M EnariS Nagata

Abstract

The homeostasis of animals is regulated not only by the growth and differentiation of cells, but also by cell death through a process known as apoptosis. Apoptosis is mediated by members of the caspase family of proteases, and eventually causes the degradation of chromosomal DNA. A caspase-activated deoxyribonuclease (CAD) and its inhibitor (ICAD) have now been identified in the cytoplasmic fraction of mouse lymphoma cells. CAD is a protein of 343 amino acids which carries a nuclear-localization signal; ICAD exists in a long and a short form. Recombinant ICAD specifically inhibits CAD-induced degradation of nuclear DNA and its DNase activity. When CAD is expressed with ICAD in COS cells or in a cell-free system, CAD is produced as a complex with ICAD: treatment with caspase 3 releases the DNase activity which causes DNA fragmentation in nuclei. ICAD therefore seems to function as a chaperone for CAD during its synthesis, remaining complexed with CAD to inhibit its DNase activity; caspases activated by apoptotic stimuli then cleave ICAD, allowing CAD to enter the nucleus and degrade chromosomal DNA.

References

Jan 1, 1992·Annual Review of Immunology·J J CohenK S Sellins
Jul 15, 1992·European Journal of Biochemistry·R WallisC Kleanthous
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 1, 1994·The Journal of Cell Biology·K Schulze-OsthoffP H Krammer
Mar 10, 1995·Science·S Nagata, P Golstein
Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Aug 1, 1993·The Journal of Cell Biology·A Batistatou, L A Greene
Aug 26, 1993·Nature·J OgasawaraS Nagata
Jan 1, 1994·Trends in Biochemical Sciences·F U HartlT Langer
Jan 1, 1993·Archives of Biochemistry and Biophysics·M A Barry, A Eastman
Jun 14, 1996·Cell·A Fraser, G Evan
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Feb 7, 1997·Cell·M D JacobsonM C Raff
Feb 7, 1997·Cell·S Nagata
Jul 1, 1997·The EMBO Journal·V L Longthorne, G T Williams
Nov 5, 1997·Archives of Biochemistry and Biophysics·D ShiokawaS Tanuma

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Citations

May 1, 1999·Journal of Medical Virology·R IchimiM Ito
Nov 21, 1998·Microscopy Research and Technique·G Gibson
Aug 14, 1999·International Journal of Cancer. Journal International Du Cancer·R MihalikL Kopper
Apr 13, 2000·International Journal of Cancer. Journal International Du Cancer·J KleeffM Korc
May 8, 2000·International Journal of Cancer. Journal International Du Cancer·H LinardouM P Deonarain
Nov 26, 1999·Journal of Cellular Biochemistry·J Y RaoG P Hemstreet
Feb 27, 1999·Journal of Cellular Physiology·P A Gregoli, M C Bondurant
Jan 7, 2000·Journal of Cellular Physiology·S RokudaiT Tsuruo
Sep 13, 2001·Journal of Biomedical Materials Research·J E Gough, S Downes
Sep 6, 2000·Journal of Cellular Biochemistry·N N KhodarevA T Vaughan
Feb 17, 2001·Journal of Cellular Biochemistry·N KiyokawaJ Fujimoto
Feb 15, 2001·Journal of Cellular Physiology·J GuJ L Nadler
Jul 19, 2002·The Anatomical Record·Wood Yee ChanDavid T Yew
Jul 10, 2001·Cell Motility and the Cytoskeleton·W M BlomJ F Nagelkerke
May 15, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A Rippe
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H C Pitot
Jan 25, 2002·International Journal of Cancer. Journal International Du Cancer·Ute A Sartorius, Peter H Krammer
Jul 13, 2002·Journal of Biomedical Materials Research·Dominique P PiolettiJacques Corbeil
Aug 14, 2001·Journal of Cellular Biochemistry·V S MujumdarS C Tyagi
Dec 18, 2001·Journal of Cellular Biochemistry·P Mlejnek
Mar 29, 2001·Bulletin of Experimental Biology and Medicine·E I DudichG T Sukhikh
Sep 24, 1999·Immunologic Research·I N Crispe
Jun 28, 2006·Chinese Journal of Integrative Medicine·Li-duan ZhengCui-huan Wu
Aug 30, 2008·Science in China. Series C, Life Sciences·B QiQ Liu
Mar 4, 2005·Archives of Pharmacal Research·Xian-Feng GongTakashi Ikejima
Sep 5, 2003·International Journal of Hematology·Dharminder ChauhanKenneth C Anderson
Jul 18, 2001·Pathology Oncology Research : POR·I Peták, J A Houghton

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