A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+ /K+ -ATPase in brain microvascular endothelial cells

The Journal of Biological Chemistry
Shu-Hong ZhangYu-Hua Chen

Abstract

Contactin-associated protein 1 (CASPR1 or CNTNAP1) was recently reported to be expressed in brain microvascular endothelial cells (BMECs), the major component of the blood-brain barrier. To investigate CASPR1's physiological role in BMECs, here we used CASPR1 as a bait in a yeast two-hybrid screen to identify CASPR1-interacting proteins and identified the β3 subunit of Na+/K+-ATPase (ATP1B3) as a CASPR1-binding protein. Using recombinant and purified CASPR1, RNAi, GST-pulldown, immunofluorescence, immunoprecipitation, and Na+/K+-ATPase activity assays, we found that ATP1B3's core proteins, but not its glycosylated forms, interact with CASPR1, which was primarily located in the endoplasmic reticulum of BMECs. CASPR1 knockdown reduced ATP1B3 glycosylation and prevented its plasma membrane localization, phenotypes that were reversed by expression of full-length CASPR1. We also found that the CASPR1 knockdown reduces the plasma membrane distribution of the α1 subunit of Na+/K+-ATPase, which is the major component assembled with ATP1B3 in the complete Na+/K+-ATPase complex. The binding of CASPR1 with ATP1B3, but not the α1 subunit, indicated that CASPR1 binds with ATP1B3 to facilitate the assembly of Na+/K+-ATPase. Furthermore, the ...Continue Reading

References

Dec 31, 1978·Life Sciences·M J Marks, N W Seeds
Aug 1, 1989·Journal of Neurochemistry·S MéresseR Cecchelli
Jan 1, 1988·Progress in Histochemistry and Cytochemistry·A W Vorbrodt
Feb 1, 1985·Experimental Neurology·H T HutchisonB Haber
Nov 8, 1984·Nature·W A JefferiesD Y Mason
Sep 13, 1996·The Journal of Biological Chemistry·N MalikR Levenson
Nov 14, 1998·The American Journal of Physiology·G Blanco, R W Mercer
Apr 15, 1999·Annual Review of Neuroscience·L L Rubin, J M Staddon
Jun 5, 2002·Annual Review of Biochemistry·Jack H Kaplan
Jul 3, 2002·The EMBO Journal·Pascal BéguinKäthi Geering
Feb 11, 2003·Advances in Experimental Medicine and Biology·Thomas GillessenStuart A Lipton
Jun 14, 2003·Brain : a Journal of Neurology·Guus Wolswijk, Rawien Balesar
Sep 13, 2003·Journal of Neurochemistry·Miroslav GottliebVivian I Teichberg
Feb 11, 2005·American Journal of Physiology. Renal Physiology·Michael RiordanNorman J Siegel
Dec 21, 2005·The Journal of Nutrition·Richard A HawkinsJuan R Viña
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erin M RoseDavid R Hampson
Aug 12, 2009·Neurobiology of Disease·N Joan AbbottDavid J Begley
Mar 5, 2010·Biochemistry·Elmira TokhtaevaOlga Vagin
Nov 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Oana CheverFlorin Amzica
Oct 5, 2012·Fundamental & Clinical Pharmacology·Li-Nan ZhangZi-Bin Gao
Dec 7, 2013·Nature Medicine·Birgit ObermeierRichard M Ransohoff
Jan 31, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joshua G JacksonMichael B Robinson
Oct 17, 2014·Journal of Neurochemistry·Holly C HunsbergerMiranda N Reed
Aug 19, 2015·The FEBS Journal·James Keaney, Matthew Campbell
Oct 29, 2015·The Journal of Physiological Sciences : JPS·Shingo Murakami, Yoshihisa Kurachi
Jun 14, 2018·Nature Communications·Wei-Dong ZhaoYu-Hua Chen

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