A catalog of human cDNA expression clones and its application to structural genomics

Genome Biology
Konrad BüssowHans Lehrach

Abstract

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

References

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Citations

Sep 20, 2005·Analytical Biochemistry·Renaud VincentelliChristophe Bignon
Jul 13, 2005·Molecular & Cellular Proteomics : MCP·Tanja FeilnerBirgit Kersten
Sep 30, 2017·Proteomics. Clinical Applications·Henry A AdeolaNonhlanhla P Khumalo
Mar 17, 2009·Biochimica Et Biophysica Acta·Jenny MattisonDavid J Adams

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Datasets Mentioned

BETA
AY243506

Methods Mentioned

BETA
size-exclusion chromatography
dynamic light scattering
differential scanning calorimetry
NMR
PCR
restriction digest
gel filtration
light scattering

Software Mentioned

Perl script
Protein Structure Factory
BLASTP
RiNA
seqjoin
MicroCal Origin
Ensembl
Perl

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