PMID: 8611600Jan 12, 1996Paper

A cation non-selective channel induced by extracellular ATP in macrophages and phagocytic cells of the thymic reticulum

Biochimica Et Biophysica Acta
R Coutinho-SilvaP M Persechini

Abstract

Extracellular ATP4- can bind to P2Z purinergic receptors including depolarization and cytoplasmic membrane permeabilization to small molecular weight solutes in macrophages, thymocytes, mast cells, phagocytic cells of the thymic reticulum and other cell types. An ATP(4-)-induced cation current has been described in whole-cell records of some of these cells but it is currently not clear whether these currents and the phenomenon of membrane permeabilization are a consequence of only one type of P2Z-associated channel/pore or two different phenomena triggered by one or more receptors. Here we use the outside-out patch-clamp technique to describe a single channel associated with this cation current in two murine phagocytic cells: intraperitoneal macrophages and phagocytic cells of the thymic reticulum. Multi channel currents could be readily observed in 77% of the outside-out patches of macrophages. Single channels of 7.8 pS could usually be resolved only in tail currents. Reversal potential measurements and ion replacement experiments indicated a lack of cation selectivity, similarly to what has already been described for the ATP(4-)-induced whole-cell inward current. No large-conductance channels that could explain the permeabili...Continue Reading

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Citations

Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Oct 3, 2014·Mediators of Inflammation·Suellen D'arc Santos OliveiraRobson Coutinho-Silva
Oct 30, 2014·Purinergic Signalling·Geoffrey Burnstock, Jean-Marie Boeynaems
Aug 1, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P M PersechiniR Coutinho-Silva
Sep 25, 2002·Physiological Reviews·R Alan North
Jan 22, 1998·The American Journal of Physiology·R Coutinho-Silva, P M Persechini
May 18, 1999·The American Journal of Physiology·R Coutinho-SilvaD M Ojcius
Dec 21, 2000·American Journal of Physiology. Cell Physiology·R Coutinho-SilvaD M Ojcius
Oct 17, 2020·Mediators of Inflammation·Maria Luiza ThorstenbergRobson Coutinho-Silva
Apr 26, 1996·Biochimica Et Biophysica Acta·R Coutinho-SilvaP M Persechini

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