A cationic nonselective stretch-activated channel in the Reissner's membrane of the guinea pig cochlea

The American Journal of Physiology
T H YehT Van den Abbeele

Abstract

The Reissner's membrane (RM) separates in the mammalian cochlea the K(+)-rich endolymph from the Na(+)-rich perilymph. The patch-clamp technique was used to investigate the transport mechanisms in epithelial cells of RM freshly dissected from the guinea pig cochlea. This study shows a stretch-activated nonselective cationic channel (SA channel) with a linear current-voltage relationship (23 pS) highly selective for cations over anions [K+ approximately Na+ (1) > Ba2+ (0.65) > Ca2+ (0.32) > Cl- (0.14)] and activated by the intrapipette gradient pressure. The open probability-pressure relationship is best fitted by a Boltzmann distribution (half-maximal pressure = 37.8 mmHg, slope constant = 8.2 mmHg). SA channels exhibit a strong voltage dependency and are insensitive to internal Ca2+, ATP, and fenamates but are blocked by 1 microM GdCl3 in the pipette. They are reversibly activated by in situ superfusion of the cell with hyposmotic solutions. Kinetic studies show that depolarization and mechanical or osmotic stretch modify the closed and open time constants probably by a different mechanism. These channels could participate in pressure-induced modifications of ionic permeability of the RM.

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Citations

Jun 15, 2006·Pflügers Archiv : European journal of physiology·Vincent CouloignerEvelyne Ferrary
Sep 14, 2007·Acta Oto-laryngologica·Masaya TakumidaMatti Anniko
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Yanggan WangBrian H Crawford
Apr 5, 2011·Journal of Neurology·Michael StruppR John Leigh
May 1, 2016·Cephalalgia : an International Journal of Headache·Doreen Huppert, Thomas Brandt
May 17, 2002·The Journal of Physiology·Alexandr V Staruschenko, Elena A Vedernikova
Dec 17, 2009·Acta Oto-laryngologica·Doreen HuppertThomas Brandt
Jan 9, 2007·The Journal of Comparative Neurology·Mitsuru SugawaraGabriel Corfas
Dec 15, 2010·Acta Oto-laryngologica·Doreen HuppertThomas Brandt
Jun 17, 2009·Expert Opinion on Pharmacotherapy·Thomas BrandtMichael Strupp
Jul 1, 2009·Therapeutic Advances in Neurological Disorders·Michael Strupp, Thomas Brandt
Jan 18, 2006·Current Opinion in Neurology·Michael Strupp, Thomas Brandt
May 30, 2001·Hearing Research·A J PaceM M Henson
Jan 11, 2012·Biochimica Et Biophysica Acta·Romain GuinamardJacques Teulon

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