PMID: 2109590Jan 1, 1990Paper

A CD 4: immunoglobulin fusion protein with antiviral effects against HIV

Archives of Virology
J P GregersenG Zettlmeissl

Abstract

Antibody-like molecules consisting of the human CD 4 extracellular domain fused to human IgG1 heavy chain constant regions were genetically constructed and expressed in a BHK cell stable transfectant. Purified chimeric antibodies bound to HIV particles as it was shown by immuno electron microscopy, inhibited fusions of HIV-1-infected cells with uninfected cells, neutralized HIV-1, and were able to inhibit the spread of a cellular HIV-1 infection in CD 4+ cells. Plaque reduction assays with CD 4(+)-transfected Hela-cells showed a comparable inhibition of HIV-1 and HIV-2. Inhibitory functions were enhanced in the presence of complement. HIV-1- and HIV-2-infected CD 4+ cells were efficiently lysed by a slow, complement-dependent mechanism, whereas uninfected CD 4+ cells and HLA-DR+ cells were not affected.

References

Apr 1, 1990·Journal of Medical Virology·J P GregersenJ Hilfenhaus
Jun 1, 1990·DNA and Cell Biology·G ZettlmeisslB Seed
Nov 28, 1986·Science·Q J SattentauP C Beverley
Feb 9, 1989·Nature·D J CaponJ E Groopman
Jan 7, 1988·Nature·R A FisherR T Schooley
Jan 7, 1988·Nature·A TrauneckerK Karjalainen
Nov 25, 1988·Science·M A TillE S Vitetta

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Citations

Nov 24, 1999·Biotechnology and Bioengineering·H J CruzM J Carrondo
Jan 1, 1994·Archives of Virology·J B Lyczak, S L Morrison
Jun 1, 1990·DNA and Cell Biology·G ZettlmeisslB Seed
Nov 7, 2014·AIDS Research and Human Retroviruses·Chun-Xia LiXiaodong Zhang
Dec 1, 1993·Revue française de transfusion et d'hémobiologie : bulletin de la Société nationale de transfusion sanguine·D Goossens

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