A cDNA-AFLP approach to study ochratoxin A production in Aspergillus carbonarius

International Journal of Food Microbiology
Alessandro BottonAngelo Ramina

Abstract

Aspergillus carbonarius is responsible for the majority of mycotoxin contaminations in grapes and its derivatives. Most of A. carbonarius strains are ochratoxin A (OTA) producers, even though at very different levels. This broad variability was used to identify genes whose expression is linked with the ability of producing OTA. A cDNA-AFLP differential display screening was performed in two strains of A. carbonarius, antagonists for the ability of producing OTA, allowing the identification of 119 differentially expressed sequences putatively involved in the regulation of OTA biosynthesis. A likely connection was pointed out between the biosynthesis of the toxin, vegetative growth and sexual/asexual developmental progression, along with common signalling pathways involving G protein and Ca2+/calmodulin dependent phosphorylation and dephoshorylation cascades.

Citations

Apr 1, 2010·Toxins·André el Khoury, Ali Atoui
Sep 23, 2014·Fungal Genetics and Biology : FG & B·Marie Foulongne-OriolJean-Michel Savoie
May 3, 2011·International Journal of Food Microbiology·A Crespo-SempereP V Martínez-Culebras
Sep 22, 2010·Molecular Plant-microbe Interactions : MPMI·Ludivine LassoisHaissam Jijakli

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