A cDNA macroarray approach to parasite-induced gene expression changes in a songbird host: genetic response of house finches to experimental infection by Mycoplasma gallisepticum

Molecular Ecology
Zhenshan WangScott V Edwards

Abstract

In 1994, the bacterial parasite Mycoplasma gallisepticum expanded its host range and swept through populations of a novel host--eastern US populations of the house finch (Carpodacus mexicanus). This epizootic caused a dramatic decline in finch population numbers, has been shown to have caused strong selection on house finch morphology, and presumably caused evolutionary change at the molecular level as finches evolved enhanced resistance. As a first step toward identifying finch genes that respond to infection by Mycoplasma and which may have experienced natural selection by this parasite, we used suppression subtractive hybridization (SSH) and cDNA macroarray approaches to identify differentially expressed genes regulated by the Mycoplasma parasite. Two subtractive cDNA libraries consisting of 16,512 clones were developed from spleen using an experimentally uninfected bird as the 'tester' and an infected bird as 'driver', and vice versa. Two hundred and twenty cDNA clones corresponding 34 genes with known vertebrate homologues and a large number of novel transcripts were found to be qualitatively up- or down-regulated genes by high-density filter hybridization. These gene expression changes were further confirmed by a high thr...Continue Reading

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Citations

Apr 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Camille BonneaudScott V Edwards
Mar 28, 2008·The Journal of Infectious Diseases·Douglas Causey, Scott V Edwards
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Nov 22, 2013·Proceedings. Biological Sciences·Lynn B MartinAaron W Schrey
Aug 19, 2011·Molecular Ecology Resources·Susan L BalengerGeoffrey E Hill

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