A cDNA microarray approach to decipher sunflower (Helianthus annuus) responses to the necrotrophic fungus Phoma macdonaldii

The New Phytologist
Marion AlignanLaurent Gentzbittel

Abstract

To identify the genes involved in the partial resistance of sunflower (Helianthus annuus) to the necrotrophic fungus Phoma macdonaldii, we developed a 1000-element cDNA microarray containing carefully chosen genes putatively involved in primary metabolic pathways, signal transduction and biotic stress responses. A two-pass general linear model was used to normalize the data and then to detect differentially expressed genes. This method allowed us to identify 38 genes differentially expressed among genotypes, treatments and times, mainly belonging to plant defense, signaling pathways and amino acid metabolism. Based on a set of genes whose differential expression was highly significant, we propose a model in which negative regulation of a dual-specificity MAPK phosphatase could be implicated in sunflower defense mechanisms against the pathogen. The resulting activation of the MAP kinase cascade could subsequently trigger defense responses (e.g. thaumatin biosynthesis and phenylalanine ammonia lyase activation), under the control of transcription factors belonging to MYB and WRKY families. Concurrently, the activation of protein phosphatase 2A (PP2A), which is implicated in cell death inhibition, could limit pathogen development....Continue Reading

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Citations

Dec 31, 2008·Journal of Bacteriology·Sivapriya Kailasan VanajaThomas S Whittam
Dec 25, 2007·BMC Genomics·Jamie A O'RourkeRandy C Shoemaker
Jun 28, 2011·Plant Signaling & Behavior·Meritxell Antolín-LloveraNarciso Campos
Oct 23, 2009·Plant Signaling & Behavior·Thierry DulermoPascale Cotton
Dec 17, 2009·Plant Signaling & Behavior·Silvia Marina PaísDaniela Andrea Capiati
Aug 21, 2007·Microbiological Research·Vijai BhadauriaYou-Liang Peng
Feb 28, 2019·Molecular Plant-microbe Interactions : MPMI·Xiao-Ren ChenWenbo Ma

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