A CE-MALDI interface based on the use of prestructured sample supports

Analytical Chemistry
T JohnsonJ Gobom

Abstract

We have developed an off-line coupling of capillary electrophoresis (CE) to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) based on CE fraction collection onto prestructured MALDI sample supports. Analyte carryover and detection sensitivity were investigated using a standard peptide mixture. Low femtomole amounts were detected, and no noticeable carryover was discovered. The performance of the method was evaluated with a mixture of tryptic digests of proteins from a human fetal brain cDNA expression library. The total number of identified peptides was increased from 47 to 211 when the CE-MALDI interface was used compared to direct MALDI-MS analysis. Sequence coverage with CE-MALDI was in the 25-60% range for the different proteins, corresponding to an increase of 1.3-4.9 times relative to that obtained with MALDI-MS of the crude mixture. Fractionation of sample components also facilitated protein identification by MALDI postsource decay analysis. Our initial results suggest this CE-MALDI interface can be used for the analysis of complex peptide mixtures isolated from biological tissues.

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Citations

Mar 30, 2004·Journal of the American Society for Mass Spectrometry·Michael J Bogan, George R Agnes
Jan 27, 2011·Analytical Chemistry·Gwendolyn J AndersonRobert T Kennedy
Sep 24, 2005·The British Journal of Nutrition·Dagmar FuchsHannelore Daniel
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Aug 28, 2001·Journal of Mass Spectrometry : JMS
Jul 30, 2014·Electrophoresis·Michael BiacchiEmmanuelle Leize-Wagner
Feb 12, 2005·Journal of Mass Spectrometry : JMS·Linda PaulsonAnn Westman-Brinkmalm
Dec 5, 2009·Electrophoresis·Michael W VannattaNorman J Dovichi
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Aug 27, 2010·Rapid Communications in Mass Spectrometry : RCM·Roza WojcikNorman J Dovichi
Dec 17, 2008·Analytical Chemistry·Francisco A Fernandez-LimaDavid H Russell
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