A Cell-Autonomous Oncosuppressive Role of Human RNASET2 Affecting ECM-Mediated Oncogenic Signaling

Cancers
Francesca RoggianiAntonella Tomassetti

Abstract

RNASET2 is an extracellular ribonuclease endowed with a marked antitumorigenic role in several carcinomas, independent from its catalytic activity. Besides its antitumorigenic role by the recruitment to the tumor mass of immune cells from the monocyte/macrophage lineage, RNASET2 is induced by cellular stress and involved in actin cytoskeleton remodeling affecting cell interactions with the extracellular matrix (ECM). Here, we aimed to investigate the effects of RNASET2 expression modulation on cell phenotype and behavior in epithelial ovarian cancer (EOC) cellular models. In silico analysis on two publicly available datasets of gene expression from EOC patients (n = 392) indicated that increased RNASET2 transcript levels are associated with longer overall survival. In EOC biopsies (n = 101), analyzed by immunohistochemistry, RNASET2 was found heterogeneously expressed among tumors with different clinical⁻pathological characteristics and, in some cases, its expression localized to tumor-associated ECM. By characterizing in vitro two models of EOC cells in which RNASET2 was silenced or overexpressed, we report that RNASET2 expression negatively affects growth capability by conferring a peculiar cell phenotype upon the interaction...Continue Reading

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Citations

Sep 25, 2019·International Journal of Molecular Sciences·Ewelina GrywalskaJacek Roliński
May 1, 2020·Molecular Plant Pathology·Naveen Kumar SinghAmnon Lers
Nov 22, 2019·Frontiers in Immunology·Francesco AcquatiDouglas M Noonan

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Methods Mentioned

BETA
transfection
flow cytometry
biopsies
immunoprecipitation
xenograft
FCS
Assay

Software Mentioned

Leica LAS AF
Adobe Photoshop
ACT
ImageJ
FlowJo
GraphPad Prism

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