PMID: 8603922Mar 1, 1996Paper

A cell cycle-associated change in Ca2+ releasing activity leads to the generation of Ca2+ transients in mouse embryos during the first mitotic division

The Journal of Cell Biology
T KonoJ Carroll

Abstract

We have used Ca2+-sensitive fluorescent dyes to monitor intracellular Ca2+ during mitosis in one-cell mouse embryos. We find that fertilized embryos generate Ca2+ transients at nuclear envelope breakdown (NEBD) and during mitosis. In addition, fertilized embryos arrested in metaphase using colcemid continue to generate Ca2+ transients. In contrast, parthenogenetic embryos produced by a 2-h exposure to strontium containing medium do not generate detectable Ca2+ transients at NEBD or in mitosis. However, when parthenogenetic embryos are cultured continuously in strontium containing medium Ca2+ transients are detected in mitosis but not in interphase. This suggests that mitotic Ca2+ transients are detected in the presence of an appropriate stimulus such as fertilization or strontium. The Ca2+ transient detected in fertilized embryos is not necessary for inducing NEBD since parthenogenetic embryos undergo nuclear envelope breakdown (NEBD). Also the first sign that NEBD is imminent occurs several minutes before the Ca2+ transient. The Ca2+ transient at NEBD appears to be associated with the nucleus since nuclear transfer experiments show that the presence of a karyoplast from a fertilized embryo is essential. Finally, we show that t...Continue Reading

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Citations

Jun 25, 1998·Current Biology : CB·K TörökM Whitaker
Apr 22, 2005·The Journal of Biological Chemistry·Rada PhilipovaMichael Whitaker
Oct 1, 1996·The Journal of Cell Biology·M WildingM Whitaker
Feb 27, 2003·Biology of Reproduction·Alexander KrivokharchenkoMichael Bader
Feb 12, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael Whitaker
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Takuya WakaiRafael A Fissore
Aug 24, 2007·Clinical and Experimental Pharmacology & Physiology·Keith T Jones
Dec 24, 2005·Physiological Reviews·Michael Whitaker
Mar 3, 2011·Reproductive Biology and Endocrinology : RB&E·Hyun YangEui-Bae Jeung
Mar 29, 2011·The Journal of Reproduction and Development·Narumi OgonukiAtsuo Ogura
May 21, 2008·The Journal of Reproduction and Development·Tomoko NakanishiTadashi Baba
Oct 30, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Sook-Young Yoon, Rafael A Fissore
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Oct 11, 2015·Cell and Tissue Research·Zoltan Machaty
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Apr 6, 2005·Biochemical and Biophysical Research Communications·Yoshie SoneShunichi Miyazaki
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Nov 3, 2010·Journal of Molecular and Cellular Cardiology·Wolfram-Hubertus Zimmermann
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Feb 13, 2001·Seminars in Cell & Developmental Biology·M Whitaker, M G Larman
Aug 16, 2000·Cell Calcium·M L DayM H Johnson

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