A cell-free model system for the study of antigen processing

International Immunology
M A Shaw, B M Chain

Abstract

Antigen processing is an essential step in the presentation of most protein antigens to class 2 MHC restricted T cells, but many of the details of processing remain unknown. In this study we show that a whole cell lysate, as well as membrane fractions from an antigen-presenting B cell lymphoma, can process ovalbumin. In this system native ovalbumin incubated with these membranes at acidic pH can be presented to an antigen-specific hybridoma by gluteraldehyde-fixed, antigen-presenting cells. This processing is inhibited by pepstatin, a selective inhibitor of aspartyl proteases. This is the first study of processing by subcellular fractions, and this cell-free system will provide a good model in which to dissect further the molecular requirements of antigen processing.

Citations

Jun 1, 1990·Immunology Today·P D King, D R Katz
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·T P Levine, B M Chain
Jul 1, 1995·European Journal of Immunology·G M Rodriguez, S Diment
Sep 24, 2005·Scandinavian Journal of Immunology·A von DelwigJ H Robinson
Nov 1, 1991·European Journal of Immunology·G KuperbergB M Chain

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.