PMID: 9168893May 12, 1997Paper

A cell-free stock of simian-human immunodeficiency virus that causes AIDS in pig-tailed macaques has a limited number of amino acid substitutions in both SIVmac and HIV-1 regions of the genome and has offered cytotropism

Virology
E B StephensO Narayan

Abstract

We have examined both the sequence changes in the LTR, gag, vif, vpr, vpx, tat, rev, vpu, env, and nef genes and the cell tropism of a cell-free stock of chimeric simian-human immunodeficiency virus (SHIV) isolated from the cerebrospinal fluid of a pig-tailed macaque (PNb) that developed AIDS. This virus (SHIVKU-1) is highly pathogenic when inoculated into other macaques. DNA sequence analysis of PCR-amplified products revealed a total of 5 nucleotide changes in the LTR while vif had 2 consensus amino acid changes. The gag, vif, and vpx had no consensus amino acid substitutions, whereas vpr had 1 consensus substitution. The tat and rev genes of the HXB2 region of SHIVKU-1 had 2 and 1 consensus amino acid changes, respectively. The vpu gene of the HXB2 region of SHIV, which originally had an ACG at the beginning of the gene, reverted to an initiation ATG codon and in addition contained a consensus amino acid substitution at position 69 of this protein. As expected, the majority of the nucleotide substitutions were found in the env and nef genes. Thirteen and 5 amino acid changes were predicted for the corresponding Env and Nef proteins, respectively. In addition, one-third of the env gene clones isolated from the SHIVKU-1 stock ...Continue Reading

References

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Nov 1, 1990·AIDS Research and Human Retroviruses·D A Regier, R C Desrosiers
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Citations

Aug 25, 2000·AIDS Research and Human Retroviruses·E B StephensV H Gattone
Apr 12, 2003·AIDS Research and Human Retroviruses·Zhihai SiJoseph Sodroski
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T L HoffmanR W Doms
Sep 12, 2015·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Qianhao XiaoKe Zhuang
Sep 25, 1998·Journal of Medical Primatology·S V JoagO Narayan
Nov 7, 2006·AIDS·Josef Vlasak, Ruth M Ruprecht
Nov 11, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Mayla HsuCecilia Cheng-Mayer
May 10, 2013·Journal of Virology·Ryan M TroyerSue VandeWoude

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