A cell initiating human acute myeloid leukaemia after transplantation into SCID mice

Nature
T LapidotJ E Dick

Abstract

Most human acute myeloid leukaemia (AML) cells have limited proliferative capacity, suggesting that the leukaemic clone may be maintained by a rare population of stem cells. This putative leukaemic stem cell has not been characterized because the available in vitro assays can only detect progenitors with limited proliferative and replating potential. We have now identified an AML-initiating cell by transplantation into severe combined immune-deficient (SCID) mice. These cells homed to the bone marrow and proliferated extensively in response to in vivo cytokine treatment, resulting in a pattern of dissemination and leukaemic cell morphology similar to that seen in the original patients. Limiting dilution analysis showed that the frequency of these leukaemia-initiating cells in the peripheral blood of AML patients was one engraftment unit in 250,000 cells. We fractionated AML cells on the basis of cell-surface-marker expression and found that the leukaemia-initiating cells that could engraft SCID mice to produce large numbers of colony-forming progenitors were CD34+ CD38-; however, the CD34+ CD38+ and CD34- fractions contained no cells with these properties. This in vivo model replicates many aspects of human AML and defines a ne...Continue Reading

References

Jan 25, 1991·Cell·C L SawyersO N Witte
May 5, 1988·The New England Journal of Medicine·M KeinänenA de la Chapelle
Jan 1, 1982·Journal of Cellular Physiology. Supplement·E A McCullochL J Smith
Dec 1, 1963·British Journal of Cancer·E H PORTER, R J BERRY

❮ Previous
Next ❯

Citations

Nov 26, 2013·Journal of Proteome Research·Cheryl F LichtiCarol L Nilsson
Jan 1, 1997·Stem Cells·A J Barrett
Jun 9, 1998·Stem Cells·D L GreinerL D Shultz
Nov 15, 2002·International Journal of Hematology·Stanley R RiddellE H Warren
Aug 29, 2012·Cellular and Molecular Life Sciences : CMLS·Huiping Liu
Sep 18, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sarah K Brennan, William Matsui
Oct 10, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Richard J Jones
Sep 29, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sara G M PiccirilloKhalid Shah
Oct 24, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Richard J Jones
Oct 5, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Boyan K Garvalov, Till Acker
May 9, 2008·European Biophysics Journal : EBJ·Vladimir P Zhdanov
Nov 26, 2010·Annals of Hematology·Ugo Testa
Jul 29, 2010·Der Pathologe·R Knüchel-ClarkeC Henkel
Nov 13, 2012·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Byung Ho KongFrederick F Lang
Feb 16, 2013·Langenbeck's Archives of Surgery·Derek ZiekerJörg Glatzle
Jan 22, 2011·Cell and Tissue Research·Ghazaleh Tabatabai, Michael Weller
Jun 22, 2012·Medical Molecular Morphology·Yoko MatsudaToshiyuki Ishiwata
Aug 6, 2009·Molecules and Cells·Deric M Park, Jeremy N Rich
Apr 8, 2010·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Yoshiro SaikawaYuko Kitagawa
Mar 8, 2012·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Yang JiangXiao-Ming Jin
Jan 20, 2011·Bulletin of Experimental Biology and Medicine·I N Shipounova NifontovaN I Drize
Oct 30, 2007·Breast Cancer Research and Treatment·Jiangbing ZhouYing Zhang
Jun 14, 2006·Cancer Metastasis Reviews·Stanley P L Leong, Marlys H Witte
Jul 25, 2007·Cancer Metastasis Reviews·Bryan C Barnhart, M Celeste Simon
Dec 17, 2009·Cancer Metastasis Reviews·William D BrandtDavid M Berman
Feb 23, 2011·Cancer Metastasis Reviews·Lesley A MathewsWilliam L Farrar
Jul 4, 2012·Cancer Metastasis Reviews·Darshni ViraEri S Srivatsan
Oct 20, 2012·Cancer Metastasis Reviews·Guerry J Cook, Timothy S Pardee
Sep 11, 2008·Cellular and Molecular Neurobiology·Xing-gang MaoHai-ning Zhen
Nov 13, 2008·Cytotechnology·Luca Vezzoni, Giorgio Parmiani
Dec 20, 2012·Cytotechnology·Shuhua ZhengYuejun Fu
Feb 27, 2009·Journal of Mammary Gland Biology and Neoplasia·Brett G HollierSendurai A Mani
May 10, 2011·Molecular and Cellular Biochemistry·Wei-Qi NianZheng-Tang Chen
May 12, 2009·Journal of Neuro-oncology·Hasan A ZaidiAlfredo Quiñones-Hinojosa
May 28, 2010·Pharmaceutical Research·Michiro SusaZhenfeng Duan
Aug 26, 2010·Targeted Oncology·Katrin Lamszus, Hauke S Günther
Jan 15, 2013·Targeted Oncology·Brian J MorrisonJason C Steel
Dec 1, 2012·Bulletin of Mathematical Biology·Thomas HillenPhilip Hahnfeldt
Jan 31, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Kai XuFuqing Zeng
Apr 29, 2010·Current Hematologic Malignancy Reports·Brian T Hill, Edward A Copelan
Jul 18, 2012·Cell Biochemistry and Biophysics·Julio Roberto Cáceres-Cortés

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.