Abstract
GJB2 mutations are the leading cause of non-syndromic inherited hearing loss. GJB2 encodes connexin-26 (CX26), which is a connexin (CX) family protein expressed in cochlea, skin, liver, and brain, displaying short cytoplasmic N-termini and C-termini. We searched for CX26 C-terminus binding partners by affinity capture and identified 12 unique proteins associated with cell junctions or cytoskeleton (CGN, DAAM1, FLNB, GAPDH, HOMER2, MAP7, MAPRE2 (EB2), JUP, PTK2B, RAI14, TJP1, and VCL) by using mass spectrometry. We show that, similar to other CX family members, CX26 co-fractionates with TJP1, VCL, and EB2 (EB1 paralogue) as well as the membrane-associated protein ASS1. The adaptor protein CGN (cingulin) co-immuno-precipitates with CX26, ASS1, and TJP1. In addition, CGN co-immunoprecipitation with CX30, CX31, and CX43 indicates that CX association is independent on the CX C-terminus length or sequence. CX26, CGN, FLNB, and DAMM1 were shown to distribute to the organ of Corti and hepatocyte plasma membrane. In the mouse liver, CX26 and TJP1 co-localized at the plasma membrane. In conclusion, CX26 associates with components of other membrane junctions that integrate with the cytoskeleton.
References
Nov 1, 1995·The Journal of Cell Biology·D Masson, T E Kreis
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
May 1, 1997·Nature·D P KelsellI M Leigh
Apr 16, 1998·The Journal of Membrane Biology·X G Wang, C Peracchia
May 28, 1998·The Journal of Biological Chemistry·T ToyofukuM Tada
Jul 1, 1998·The Journal of Cell Biology·M FuruseS Tsukita
Aug 26, 1998·Current Biology : CB·B N Giepmans, W H Moolenaar
Dec 3, 1999·Biochemical and Biophysical Research Communications·T KojimaD C Spray
Dec 30, 1999·The Journal of Cell Biology·M CordenonsiS Citi
Jul 11, 2000·The Journal of Biological Chemistry·A NusratR J Mrsny
Feb 13, 2001·Experimental Cell Research·T KojimaN Sawada
Feb 13, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y F PengY Takai
Apr 9, 2001·Nitric Oxide : Biology and Chemistry·B R FlamD C Eichler
Sep 15, 2001·FEBS Letters·P J KausalyaW Hunziker
May 1, 2002·Biochemistry·Anne-Lane SchubertMichael P Lisanti
Jul 5, 2002·Audiology & Neuro-otology·Philine Wangemann
Oct 31, 2002·Analytical Chemistry·Andrew KellerRuedi Aebersold
Mar 28, 2003·Analytical Chemistry·Jan HavlisAndrej Shevchenko
Jun 17, 2003·Molecular Biology of the Cell·Peter A NielsenNalin M Kumar
Apr 20, 2004·The European Journal of Neuroscience·Xinbo LiJames I Nagy
Apr 28, 2004·Hearing Research·Michael T HenzlRuediger Thalmann
Dec 14, 2004·Nature Cell Biology·Martina BeltramelloFabio Mammano
Jun 28, 2005·The Journal of Biological Chemistry·Deepika SinghPaul D Lampe
Jul 28, 2005·The European Journal of Neuroscience·Mihai C PenesJames I Nagy
Sep 9, 2005·Molecular Biology of the Cell·Yulia KomarovaAnna Akhmanova
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yanping ZhangXi Lin
Jun 3, 2006·Circulation Research·Antonio Rodriguez-SinovasDavid Garcia-Dorado
Aug 18, 2006·Development·Xin XuCecilia W Lo
Dec 23, 2006·American Journal of Human Genetics·Saima RiazuddinThomas B Friedman
Feb 10, 2007·Cell·Robin M ShawLily Y Jan
Mar 28, 2007·Biochemical and Biophysical Research Communications·Rieko AjimaJun Yokota
May 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rick F NelsonHenry L Paulson
Jul 7, 2007·American Journal of Physiology. Cell Physiology·Sabrina W YumSteven S Scherer
Jul 31, 2007·Genetics·Samin A SajanMichael Lovett
Mar 28, 2008·Biochemical Society Transactions·Wolfgang H ZieglerJonas Emsley
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Carmen E FloresAlberto E Pereda
Dec 3, 2008·Biochimica Et Biophysica Acta·Mickaël DerangeonJean-Claude Hervé
Mar 17, 2009·Cell Communication and Signaling : CCS·Hashem A DboukRabih S Talhouk
Mar 25, 2009·BMC Neuroscience·Yoko Shiraishi-YamaguchiTeiichi Furuichi
Apr 3, 2009·Nature·Shoji MaedaTomitake Tsukihara
Aug 22, 2009·Annual Review of Genetics·Amiel A Dror, Karen B Avraham
Sep 22, 2009·Biochemical and Biophysical Research Communications·Pablo M PeixotoKathleen W Kinnally
Oct 30, 2009·Human Molecular Genetics·Francisco J del CastilloChristine Petit
Dec 1, 2009·Trends in Cell Biology·Dale W Laird
Dec 27, 2011·Biochimica Et Biophysica Acta·Jean-Claude HervéNicolas Bourmeyster
Mar 13, 2012·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Halina S ChkourkoMario Delmar
Mar 20, 2012·Nature·Thomas MetzgerMary K Baylies
Oct 10, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Simon AngeliXue Zhong Liu
Mar 5, 2013·Physiology·Anastasia F ThéveninMatthias M Falk
Apr 18, 2013·Journal of Proteome Research·Stephanie L FowlerSteffany A L Bennett