A cell mutant that exhibits temperature-dependent sensitivity to transformation by various oncogenes.

Molecular and Cellular Biology
S Kizaka, A Hakura

Abstract

We previously isolated a Fisher rat fibroblast mutant, B812, that has the unique property of temperature-dependent transformation by various oncogenic retroviruses. At the permissive temperature (35 degrees C), this mutant was sensitive to oncogenic transformation and formed foci on a dish at the same frequency as did the parental fibroblast cell line. When Kirsten murine sarcoma virus (Ki-MSV) was applied to the cells, the frequency of focus formation decreased more than 25-fold at the nonpermissive temperature (39 degrees C), whereas the cells expressed nearly the same level of the ras transcript as well as the ras protein. The temperature-restricted focus formation was fully reversible and was completely suppressed upon fusion with the wild-type parent cell. In addition to ras, the mos, fos, src, and erbB-2 oncogenes transformed this mutant with the same temperature dependence as described above; polyomavirus middle T antigen, adenovirus type 12, and human papillomavirus 16-E67 also transformed, but without temperature dependence. These results suggest that ras, fos, mos, src, and erbB-2 use a common cellular pathway for transforming cells.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A F LauA J Faras
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Nakajima-IijimaT Kakunaga
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C SetoyamaB de Crombrugghe
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K ToyoshimaT Yamamoto
Jan 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H C Renger, C Basilico
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M EdmondsH Nakazato
Feb 1, 1983·Molecular and Cellular Biology·H Okayama, P Berg
Nov 18, 1983·Science·H LandR A Weinberg
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W S KloetzerR B Arlinghaus
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M NodaR H Bassin

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Citations

May 8, 1992·International Journal of Cancer. Journal International Du Cancer·L A HeathF Propst
Feb 11, 2009·Molecular Carcinogenesis·Masaaki TatsukaTakashi Takata
Jul 20, 2005·Oncogene·Akifumi KandaMasaaki Tatsuka
Aug 1, 1990·Molecular and Cellular Biology·A Engelman, N Rosenberg

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