PMID: 9450262Feb 5, 1998Paper

A cell perifusion system of isolated rat islets for studies on rapid insulin-glucose dynamics

Medical Engineering & Physics
N K YaoL W Chang

Abstract

We have developed a cell perifusion system of isolated rat islets, to improve the accuracy of controlling the medium content and the stability of the medium flow. This new system presents the following characteristics: (1) There is always a consistent flow in the perifusion chamber and only half of its volume is altered when switching the medium. (2) The medium containing test substances can be prepared and held ready in very close proximity to islets. The test glucose media are introduced to the islets immediately after a switch. (3) The environment of the islets could be repeatedly changed from one state to another in a rigorous stepwise manner. The system can provide an accurate switch of glucose concentrations both in time and quantity without introducing pressure disturbances, which makes a suitable tool for studies on rapid insulin-glucose dynamics. In experiments of perifused rat islets, our results further confirmed the observation that the amplitudes of oscillatory insulin secretions were magnified when glucose levels increased but their periods were unaffected. In addition, insulin secretions instantly increased in response to the sudden increase of glucose levels, but gradually decreased in response to the sudden dec...Continue Reading

References

Jun 1, 1991·Endocrinology·M Hiriart, M C Ramirez-Medeles
Jan 1, 1990·Diabetes·H F Chou, E Ipp
Dec 1, 1984·Computers and Biomedical Research, an International Journal·M NomuraH Abe

❮ Previous
Next ❯

Citations

May 7, 1998·Journal of Biomedical Materials Research·T H YoungL W Chen
Apr 1, 2001·Cell Transplantation·Jyuhn-Huarng JuangNan-Kuang Yao
Mar 27, 2001·Transplantation Proceedings·J H JuangN K Yao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.