Jun 14, 2016

A cell type-specific expression signature predicts haploinsufficient autism-susceptibility genes

BioRxiv : the Preprint Server for Biology
Chaolin Zhang, Yufeng Shen

Abstract

Recent studies have identified many genes with rare de novo mutations in autism, but a limited number of these have been conclusively established as disease-susceptibility genes due to lack of recurrence and confounding background mutations. Such extreme genetic heterogeneity severely limits recurrence-based statistical power even in studies with a large sample size. In addition, the cellular contexts in which these genomic lesions confer disease risks remain poorly understood. Here we investigate the use of cell-type specific expression profiles to differentiate mutations in autism patients or unaffected siblings. Using 24 distinct cell types isolated from the mouse central nervous system, we identified an expression signature shared by genes with likely gene disrupting (LGD) mutations detected by exome-sequencing in autism cases. The signature reflects haploinsufficiency of risk genes enriched in transcriptional and post-transcriptional regulators, with the strongest positive associations with specific types of neurons in different brain regions, including cortical neurons, cerebellar granule cells, and striatal medium spiny neurons. Based on this signature, we assigned a D score to all human genes to prioritize candidate aut...Continue Reading

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Mentioned in this Paper

Study
CNS (Mmhcc)
Post-Transcriptional Regulation
Genome
Genes
Transcription, Genetic
Neurons
Intolerance Function
Recurrent Malignant Neoplasm
Gene Mutation

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