Jul 14, 2001

A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA

Science
G HutvágnerPhillip D Zamore

Abstract

The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

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Mentioned in this Paper

Biochemical Pathway
RNA Conformation
Transfection
Caenorhabditis elegans
Primary RNA Transcript
RNA, Double-Stranded
Transcription, Genetic
Tertiary Protein Structure
RNA, Small Temporal
RNA Precursor

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