A cellular model system of differentiated human myotubes

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
M GasterH D Schrøder

Abstract

The aim of this study was to select an effective and stable protocol for the differentiation of human satellite cells (Sc) and to identify the optimal time period for the experimental use of differentiated human Sc-cultures. In order to identify the differentiation conditions which give a good survival of myotubes and a high grade of differentiation, Sc-cultures were induced to differentiate in media supplemented with either 2% fetal calf serum (FCS) 2% horse serum (HS) or 10% HS. Based on higher CK-activities in cultures differentiating in FCS-supplemented media compared to horse sera, fetal calf serum was chosen to induce differentiation. The ATP, DNA and protein content increased during the first 4 days after induction of differentiation and was followed by a period with minor changes. The maximal differences of ATP, DNA and protein between days 4-10 were evaluated and the differences in the three components were found to be less than 20% of the average value with a certainity of more than 0.9. Day 8-myotubes were investigated morphologically and were found immunoreactive for fast myosin, and expressed areas with clear cross striation. We recommend the use of differentiated Sc-cultures in the period from day 4 to 8 after ind...Continue Reading

Citations

Jun 12, 2013·Cell and Tissue Research·Vigdis AasArild C Rustan
Sep 10, 2003·Best Practice & Research. Clinical Endocrinology & Metabolism·Henning Beck-NielsenMichael Gaster
Mar 11, 2011·BMC Physiology·Marissa K TrenerryDavid Cameron-Smith
Apr 7, 2010·Journal of Lipid Research·N P HessvikG H Thoresen
Jan 15, 2014·PloS One·Robert SmithHarald Thidemann Johansen
May 31, 2012·Stem Cell Reviews and Reports·Mandana Haack-SørensenJens Kastrup
Feb 11, 2009·The Journal of Physiology·Victoria C FolettaKen R Walder
Dec 29, 2010·Biochemical and Biophysical Research Communications·Michael Gaster
Oct 6, 2010·Biochemical and Biophysical Research Communications·Ariane D Minet, Michael Gaster
Dec 23, 2009·Biochemical and Biophysical Research Communications·Michael Gaster
Jul 21, 2009·Biochemical and Biophysical Research Communications·Michael Gaster
Oct 28, 2008·Biochimica Et Biophysica Acta·Michael Gaster
Feb 3, 2007·Molecular and Cellular Endocrinology·P CavuotoG A Wittert
Sep 24, 2005·European Journal of Clinical Investigation·B M AbdallahM Gaster
Oct 13, 2015·Methods : a Companion to Methods in Enzymology·Mark JuhasNenad Bursac
Oct 16, 2007·Biochemical and Biophysical Research Communications·Paul CavuotoGary A Wittert
Jun 15, 2004·Biochemical and Biophysical Research Communications·Michael GasterHenning Beck-Nielsen
Jan 31, 2006·Biochimica Et Biophysica Acta·Michael Gaster, Henning Beck-Nielsen
Mar 28, 2009·Biochemical and Biophysical Research Communications·Michael Gaster
Oct 24, 2014·Journal of Cellular and Molecular Medicine·Pascal PomièsMaurice Hayot
Feb 1, 2014·European Journal of Medicinal Chemistry·Ove Alexander Høgmoen ÅstrandEili Tranheim Kase
Jun 15, 2014·Virology·Masaharu IwasakiJuan C de la Torre
Nov 5, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Nina Pettersen HessvikEili Tranheim Kase
May 10, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·G H ThoresenA C Rustan

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