PMID: 9557674Apr 29, 1998Paper

A cellular protein binds vaccinia virus late promoters and activates transcription in vitro

Journal of Virology
M ZhuSteven S Broyles

Abstract

Available evidence indicates that the transcription of the late class of vaccinia virus genes requires the participation of several virus-encoded proteins in addition to the viral RNA polymerase. In this report we describe the identification of a protein present in extracts of uninfected HeLa cells that binds avidly to viral late promoter DNA. The protein bound specifically to several different vaccinia virus late promoters but not an early nor an intermediate promoter. DNase I footprinting localized the protein's binding site to nucleotides surrounding the transcriptional start site of the I1L promoter. Optimal promoter binding required sequences in the highly conserved TAAAT motif at the transcriptional start site as well as sequences immediately upstream; however, one variation on the motif's sequence did not affect promoter binding by the protein. Partially purified late promoter binding protein (LPBP) was capable of stimulating the transcription activity of extracts depleted of LPBP on a late promoter-driven template, establishing LPBP as a transcription activator in vitro. These results suggest that a cellular protein is responsible for targeting vaccinia virus late promoters for initiation of transcription.

References

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Citations

Mar 30, 2001·The Journal of Biological Chemistry·M R Mohamed, E G Niles
Jun 4, 2004·Expert Opinion on Biological Therapy·Z Sheng Guo, David L Bartlett
Apr 12, 2015·Journal of Laboratory Automation·Christopher F Fronczek, Jeong-Yeol Yoon
Dec 10, 1999·The Journal of Biological Chemistry·S S BroylesM Kremer
Mar 20, 2009·The Journal of General Virology·Bruce A KnutsonSteven S Broyles
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·P Sanz, B Moss
Oct 9, 1998·The Journal of Biological Chemistry·S K GunasingheC F Wright
Jan 8, 2000·The Journal of Biological Chemistry·C A Lackner, R C Condit
Sep 6, 2002·Biochimica Et Biophysica Acta·Richard C Condit, Edward G Niles
Jun 23, 2004·Virology·Mohamed Ragaa Mohamed, Edward G Niles

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