PMID: 9428674Jan 15, 1998Paper

A central role for water in the control of the spin state of cytochrome P-450scc

European Journal of Biochemistry
F BancelR Lange

Abstract

A previous thermodynamic study [Lange, R., Larroque, C. & Anzenbacher, P. (1992) Eur. J. Biochem. 207, 69-73] demonstrated two conformations (A and B) of cytochrome P-450scc (SCC), the enzyme which initiates steroid biosynthesis by cleaving the side chain of cholesterol. The conformation found at the lowest temperatures (form A) displays a six-ligand high-spin heme iron [Hildebrandt, P., Heibel, G., Anzenbacher, P., Lange, R., Krüger, V. & Stier, A. (1994) Biochemistry 33, 12920-12929]. Analytical centrifugation shows that the oligomeric composition of SCC is the same for the A and the B conformers. However, as revealed by fourth-derivative ultraviolet spectroscopy, the two conformers differ in the mean environment of the tryptophan residues, which was more polar in the A form. The structural role of water in these two conformations was investigated using the pressure-jump technique under various pH, temperature and osmotic-stress conditions. Applying hydrostatic pressure to SCC induced very slow (tau >30 min) biexponential relaxation kinetics corresponding to the high-spin to low-spin transition. Analysis of the activation volumes suggested a dissociative mechanism for the A conformer (+45 ml/mol), and an associative mechanism...Continue Reading

References

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Citations

Nov 25, 2003·Biochemical and Biophysical Research Communications·Dmitri R DavydovGaston Hui Bon Hoa
May 2, 2002·Biochimica Et Biophysica Acta·Reinhard Lange, Claude Balny
May 2, 2002·Biochimica Et Biophysica Acta·Christiane Jung
Apr 27, 2001·European Journal of Biochemistry·K RuanC Balny
Jan 19, 2012·Antimicrobial Agents and Chemotherapy·Andrew G S WarrilowSteven L Kelly
Oct 12, 2000·Biochemical and Biophysical Research Communications·D R DavydovG H Hoa
Dec 2, 2008·Expert Opinion on Drug Metabolism & Toxicology·Dmitri R Davydov, James R Halpert
Mar 15, 2011·Expert Opinion on Drug Metabolism & Toxicology·Dmitri R Davydov
Feb 17, 2006·Biochimica Et Biophysica Acta·Joan TorrentReinhard Lange
Oct 24, 2002·Archives of Biochemistry and Biophysics·Madeleine J Headlam, Robert C Tuckey
May 16, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·D R Davydov
Apr 17, 2013·Biotechnology and Applied Biochemistry·Dmitri R DavydovJames R Halpert

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