A centrosomal protein STARD9 promotes microtubule stability and regulates spindle microtubule dynamics

Cell Cycle
Shalini Srivastava, Dulal Panda

Abstract

Centrosomal proteins play important roles in the spindle assembly and the segregation of chromosomes in the eukaryotic cells. STARD9, a recently identified centrosomal protein, was reported to influence the spindle pole assembly. However, the role of STARD9 in maintaining the stability and organization of microtubules are not known. Here, we show that STARD9 regulates the assembly and dynamics of both interphase and mitotic microtubules. The knockdown of STARD9 in HeLa or HCT116 cells with siRNA or shRNA induced a strong depolymerization of the interphase microtubules. The over-expression of the motor domain of STARD9 stabilizes microtubules against cold and nocodazole suggesting that STARD9 stabilizes microtubules in HeLa cells. Using fluorescent recovery after photobleaching, we showed that the knockdown of STARD9 strongly reduced microtubule dynamics in the live spindles of HeLa cells. The reassembly of microtubules in the STARD9-depleted cells was strongly reduced as compared to the microtubules in the control cells implying the role of STARD9 in the nucleation of microtubules. Further, the depletion of STARD9 inhibited chromosome separation and the STARD9-depleted HeLa cells were blocked at mitosis. Interestingly, the freq...Continue Reading

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
May 11, 2001·The Oncologist·J M Nabholtz, A Riva
Jan 17, 2002·Current Opinion in Cell Biology·Michel Bornens
Sep 6, 2002·The Journal of Cell Biology·Stephen L RogersRonald D Vale
Oct 31, 2002·The Journal of Cell Biology·Alexander Dammermann, Andreas Merdes
Jul 25, 2003·The Journal of Biological Chemistry·Bernd GieseGerhard Müller-Newen
Oct 6, 2005·Cell Motility and the Cytoskeleton·Gerard W DoughertyBechara Kachar
Nov 24, 2005·Current Biology : CB·Gohta GoshimaRonald D Vale
Sep 19, 2006·Nature Cell Biology·Naoki OshimoriTadashi Yamamoto
Jan 26, 2007·Nature Reviews. Cancer·Jeffrey R JacksonPearl S Huang
Jun 8, 2007·Molecular Biology of the Cell·Daniel W BusterDavid J Sharp
Feb 26, 2008·The Biochemical Journal·Ko MomotaniAvril V Somlyo
Oct 29, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio JimenoRoss C Donehower
Jan 23, 2009·Molecular Biology of the Cell·Rania S RizkClaire E Walczak
Jan 27, 2009·Current Protocols in Neuroscience·R Benjamin FreeDavid R Sibley
May 12, 2009·Current Biology : CB·Steffen LawoLaurence Pelletier
Nov 13, 2009·Current Biology : CB·Sophie Dumont, Timothy J Mitchison
Apr 14, 2010·Cancer Chemotherapy and Pharmacology·Anne M TraynorGeorge Wilding
Aug 5, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ralf-Dieter HofheinzGerd Munzert
Aug 28, 2010·Current Biology : CB·Harjinder S SardarSusan P Gilbert
Oct 26, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cristhiaan D OchoaRon Balczon
Jul 15, 2011·Methods in Molecular Biology·Hoi Tang Ma, Randy Y C Poon
Jan 17, 2012·Current Opinion in Cell Biology·Robert Mahen, Ashok R Venkitaraman
Jun 21, 2012·PloS One·Venkataramana K SidhayeLandon S King
Dec 17, 2014·Molecular Biology of the Cell·Silvia SeneseJorge Z Torres
Mar 20, 2015·Expert Opinion on Therapeutic Targets·Jubina B VenghateriDulal Panda
Oct 7, 2015·Nature Communications·Yalei Chen, William O Hancock
Mar 13, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hauke Drechsler, Andrew D McAinsh
May 8, 2018·IUBMB Life·Anuradha Kumari, Dulal Panda

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Citations

Dec 11, 2020·Cell Death Discovery·Francesca CastoldiFederico Pietrocola
Jan 14, 2020·Molecular and Cellular Endocrinology·Barbara J Clark

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Methods Mentioned

BETA
immunoprecipitation assay
pull down
transfection
confocal microscopy
immunoprecipitation
fluorescence recovery after photobleaching

Software Mentioned

Zen
GraphPad Prism
Excel
ImageJ

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