Abstract
Cell-mediated immune responses constitute a major defense against the spread of human immunodeficiency virus type 1 (HIV-1). However, multiple alterations within a particular epitope may accumulate during disease progression, allowing the virus to escape cytotoxic T lymphocytes (CTLs). Therefore, the best candidate for a peptide vaccine that would prevent the onset of the disease might be a chain section containing epitopes for the generation of CTLs in regions of conserved sequences among different HIV-1 isolates. We previously showed that immunizing mice with synthetic peptides consisting of 23-amino acids (Gag-23mer; 287-309 amino acid residues) in a highly conserved region derived from the major core protein p24 of HIV-1 generates specific CTLs as well as antibodies. Here, we identified one CTL (T-1; 291-300) and two B-cell (B-1; 290-299 and B-2; 300-309) epitopes, all of which consisted of 10 amino acids within the region. In addition, helper T cells primed by the Gag-23mer peptide were proliferated by in vitro stimulation with a 21mer (H-1; 289-309) or a 19mer (H-2; 291-309) peptide, but not with a 17mer peptide (293-309) or 19mer peptide (287-305). Immunization with the H-1 peptide generated an antibody reactive to B-1, ...Continue Reading
References
Dec 1, 1978·International Journal of Cancer. Journal International Du Cancer·D J MossJ H Pope
Jun 1, 1992·Virology·K J SastryR B Arlinghaus
Jun 26, 1992·Science·D F HuntA Sette
Jan 1, 1992·Vaccine·Y NishinoK Ikuta
Sep 1, 1992·The Journal of General Virology·K MatsuoK Ikuta
Mar 6, 1992·Science·D F HuntV H Engelhard
Mar 1, 1990·AIDS·B D Walker, F Plata
May 1, 1990·Molecular Immunology·J HinkulaB Wahren
Nov 15, 1990·Nature·G M Van Bleek, S G Nathenson
May 23, 1991·Nature·K FalkH G Rammensee
Sep 26, 1991·Nature·T S JardetzkyD C Wiley
Jan 25, 1991·Cell·J M McCune
Apr 9, 1987·Nature·A R CoatesM J Sternberg
Dec 1, 1988·Nature·D F NixonA J McMichael
Jun 1, 1989·Virology·K IkutaS Kato
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K JavaherianS D Putney
Jul 7, 1983·The New England Journal of Medicine·A J McMichaelP A Beare
Jan 1, 1993·AIDS·A Venet, B D Walker
Apr 1, 1995·The Journal of Experimental Medicine·M R KleinF Miedema
Mar 1, 1995·AIDS Research and Human Retroviruses·Y CaoM S Saag
May 1, 1994·Vaccine·Y NishinoK Ikuta
Aug 11, 1994·Nature·G PantaleoR P Sekaly
Feb 1, 1993·The Journal of Experimental Medicine·A CarmichaelL Borysiewicz
Feb 1, 1994·Vaccine·M KameokaK Ikuta
Jun 1, 1993·AIDS·J LouwagieR Deleys
Sep 1, 1995·Trends in Microbiology·A McKnight, P R Clapham
Oct 1, 1995·Trends in Microbiology·M R Klein, F Miedema
Mar 1, 1996·AIDS Research and Human Retroviruses·C TruongF Barin
Citations
Apr 23, 2003·Vaccine·Yurie NakayaAdolfo García-Sastre
Jun 7, 2008·Human Gene Therapy·Diana HammerRalf Wagner
Jan 12, 2000·Viral Immunology·J ArpG Dekaban
Jan 27, 2005·Viral Immunology·Sanjeev Kumar, Pradeep Seth
Aug 20, 2010·Journal of Virology·April L FerreBarbara L Shacklett
Feb 22, 2001·The Journal of Clinical Investigation·U MalhotraM J McElrath
Aug 11, 2010·BMC Microbiology·Sinu Paul, Helen Piontkivska
Dec 3, 2003·Journal of Immune Based Therapies and Vaccines·Lokesh AgrawalD Nageswara Rao
Aug 8, 2013·PloS One·Robert D Bremel, E Jane Homan
Aug 21, 2007·Journal of Molecular Recognition : JMR·Adriana SoutulloGeorgina G Tonarelli
Sep 13, 2005·Vaccine·Alexandra FluriGiuseppe Bertoni
May 9, 2000·Clinical and Diagnostic Laboratory Immunology·J IroegbuM Sällberg
Feb 5, 2004·World Journal of Gastroenterology : WJG·Chang-Zheng SongZeng-Liang Bai
Sep 11, 2004·Viral Immunology·Priti Chugh, Pradeep Seth
Apr 10, 1999·Journal of Virology·S M LonningT C McGuire
Mar 12, 1999·Journal of Virology·S M LonningT C McGuire
Sep 11, 1999·Journal of Virology·P S PolacinoS L Hu
Nov 13, 1998·Journal of Virology·W ZhangT C McGuire