A Change in a Single Midgut Receptor in the Diamondback Moth (Plutella xylostella) Is Only in Part Responsible for Field Resistance to Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai.

Applied and Environmental Microbiology
Denis J WrightJ Ferre

Abstract

A population (SERD3) of the diamondback moth (Plutella xylostella L.) with field-evolved resistance to Bacillus thuringiensis subsp. kurstaki HD-1 (Dipel) and B. thuringiensis subsp. aizawai (Florbac) was collected. Laboratory-based selection of two subpopulations of SERD3 with B. thuringiensis subsp. kurstaki (Btk-Sel) or B. thuringiensis subsp. aizawai (Bta-Sel) increased resistance to the selecting agent with little apparent cross-resistance. This result suggested the presence of independent resistance mechanisms. Reversal of resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai was observed in the unselected SERD3 subpopulation. Binding to midgut brush border membrane vesicles was examined for insecticidal crystal proteins specific to B. thuringiensis subsp. kurstaki (Cry1Ac), B. thuringiensis subsp. aizawai (Cry1Ca), or both (Cry1Aa and Cry1Ab). In the unselected SERD3 subpopulation (ca. 50- and 30-fold resistance to B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai), specific binding of Cry1Aa, Cry1Ac, and Cry1Ca was similar to that for a susceptible population (ROTH), but binding of Cry1Ab was minimal. The Btk-Sel (ca. 600-and 60-fold resistance to B. thuringiensis subsp. k...Continue Reading

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Citations

Jun 27, 2001·Applied and Environmental Microbiology·Y B LiuN Crickmore
Nov 19, 2003·Pest Management Science·Ali H SayyedDenis J Wright
May 1, 1999·Applied and Environmental Microbiology·V BallesterJ Ferre
Dec 1, 2001·Annual Review of Entomology·Juan Ferré, Jeroen Van Rie
Dec 11, 2008·Annual Review of Entomology·Aaron J GassmannBruce E Tabashnik
Sep 26, 2001·Applied and Environmental Microbiology·J González-CabreraJ Ferré
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Craig R Pigott, David J Ellar
Mar 7, 2001·Applied and Environmental Microbiology·S HerreroJ Ferré
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·B E TabashnikJ Ferré
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Jun 5, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masahiro HiguchiHidetaka Hori
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