PMID: 9874766Jan 6, 1999

A change in actin conformation associated with filament instability after Pi release

Proceedings of the National Academy of Sciences of the United States of America
L D BelmontEdward H Egelman

Abstract

The ability of actin to both polymerize into filaments and to depolymerize permits the rapid rearrangements of actin structures that are essential for actin's function in most cellular processes. Filament polarity and dynamic properties are conferred by the hydrolysis of ATP on actin filaments. Release of inorganic phosphate (Pi) from filaments after ATP hydrolysis promotes depolymerization. We identify a yeast actin mutation, Val-159 to Asn, which uncouples Pi release from the conformational change that results in filament destabilization. Three-dimensional reconstructions of electron micrographs reveal a conformational difference between ADP-Pi filaments and ADP filaments and show that ADP V159N filaments resemble ADP-Pi wild-type filaments. Crystal structures of mammalian beta-actin in which the nucleotide binding cleft is in the "open" and "closed" states can be used to model actin filaments in the ADP and ADP-Pi conformations, respectively. We propose that these two conformations of G-actin may be related to two functional states of F-actin.

References

Oct 20, 1992·Journal of Molecular Biology·A Orlova, Edward H Egelman
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M FlahertyK C Holmes
Jan 1, 1991·Molecular and Cellular Biology·R KarlssonA S Byström
Jan 1, 1990·Advances in Biophysics·Marie-France Carlier
Feb 1, 1990·Current Opinion in Cell Biology·Thomas D Pollard
Sep 6, 1990·Nature·W KabschK C Holmes
Sep 6, 1990·Nature·K C HolmesW Kabsch
Jul 28, 1989·Science·Edward H Egelman, X Yu
Mar 23, 1987·FEBS Letters·M CouéE D Korn
Aug 5, 1987·Biochimica Et Biophysica Acta·M Wanger, A Wegner
Jun 26, 1981·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T A SteitzW S Bennett
Apr 1, 1995·Current Opinion in Structural Biology·Edward H Egelman, A Orlova
Jul 20, 1993·Journal of Molecular Biology·A Orlova, Edward H Egelman
Oct 28, 1993·Nature·Clarence E SchuttUno Lindberg
Jul 15, 1996·The Biochemical Journal·J MoraczewskaC G dos Remedios
Jan 1, 1996·Annual Review of Biophysics and Biomolecular Structure·J H Hurley
Nov 8, 1996·Journal of Molecular Biology·John K ChikClarence E Schutt
Jul 3, 1997·Nature·P Lappalainen, David G Drubin
Aug 15, 1997·Journal of Molecular Biology·A OrlovaEdward H Egelman
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R Dyche MullinsThomas D Pollard
Jul 17, 1998·Journal of Molecular Biology·R PageClarence E Schutt
Sep 10, 1998·The Journal of Cell Biology·L D Belmont, David G Drubin
Jun 1, 1996·Trends in Cell Biology·R Dyche MullinsThomas D Pollard

Citations

Jul 6, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L A Boyer, C L Peterson
Dec 14, 2002·Angewandte Chemie·Kap-Sun Yeung, Ian Paterson
May 7, 2010·Molecules and Cells·Sung Haeng Lee, Roberto Dominguez
Nov 19, 2003·Journal of Molecular Biology·John K Chik, David C Schriemer
Nov 3, 2001·Biochimica Et Biophysica Acta·Herwig Schüler
Jan 19, 2000·Trends in Biochemical Sciences·H ChenJames R Bamburg
Sep 18, 2007·Nature Structural & Molecular Biology·A OrlovaEdward H Egelman
Jun 24, 2000·European Journal of Biochemistry·Herwig SchülerR Karlsson
Feb 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·V E GalkinEdward H Egelman
Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jim PfaendtnerGregory A Voth
Apr 24, 2009·The Journal of Biological Chemistry·Kuo-Kuang Wen, Peter A Rubenstein
Nov 26, 2009·The Journal of Biological Chemistry·Taro Q P NoguchiTaro Q P Uyeda
Jan 29, 2010·The Journal of Biological Chemistry·David PoppRobert C Robinson
Sep 10, 2010·The Journal of Biological Chemistry·Stephan SchmitzClaudia Veigel
Jan 29, 2008·The Journal of Biological Chemistry·Kuo-Kuang WenPeter A Rubenstein
Jun 3, 2008·The Journal of Biological Chemistry·Mitsusada IwasaToshiro Oda
May 15, 2008·The Journal of Biological Chemistry·Becky M Miller, Kathleen M Trybus
Dec 12, 2002·Molecular Biology of the Cell·Guido PosernRichard Treisman
Jun 25, 2005·Molecular Biology of the Cell·Sean W Clark, Mark D Rose
Oct 1, 2005·Molecular Biology of the Cell·Jean MullerBarbara Winsor
Dec 18, 2002·The EMBO Journal·Fusinita van den EntJan Löwe
May 30, 2009·Human Molecular Genetics·Matías MorínMiguel Angel Moreno-Pelayo
Aug 8, 2009·Biological Reviews of the Cambridge Philosophical Society·Iryna MonastyrskaFulvio Reggiori
Mar 3, 2010·Annual Review of Biophysics·Beáta Bugyi, Marie-France Carlier
Mar 30, 2007·BMC Genomics·Ivonne M SehringRoland Kissmehl
Nov 20, 2013·Trends in Cell Biology·Prabodh Kapoor, Xuetong Shen
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ikuko FujiwaraThomas D Pollard
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dimitrios VavylonisBen O'Shaughnessy
Sep 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jhih-Wei Chu, Gregory A Voth
Aug 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elena P SablinRobert J Fletterick
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J DayelR Dyche Mullins
May 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·S VorobievS C Almo
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mark I R PetalcorinSimon J Boulton
Nov 5, 2014·Neural Regeneration Research·Ailin DuRuiling Zhang
Dec 6, 2003·The Journal of Cell Biology·V E GalkinEdward H Egelman
Jan 20, 2005·The Journal of Cell Biology·Adam C MartinDavid G Drubin
Sep 19, 2007·The Journal of Cell Biology·Voytek Okreglak, David G Drubin
Aug 23, 2000·The Journal of Cell Biology·A K WolvenDavid G Drubin
Jul 21, 2015·Current Biology : CB·Dennis ZimmermannRonald S Rock
Jan 25, 2011·European Journal of Cell Biology·Julia Magdalena SattlerHerwig Schüler
Nov 26, 2009·Journal of Molecular Biology·Jim PfaendtnerGregory A Voth
Dec 24, 2014·Structure·V E GalkinEdward H Egelman
Mar 3, 2005·EMBO Reports·Adeleke H AgudaRobert C Robinson
Jun 25, 2004·The EMBO Journal·Leslie D BurtnickRobert C Robinson
Oct 15, 2005·IUBMB Life·Peter A Rubenstein, Kuo-Kuang Wen
May 13, 2006·The FEBS Journal·Andras MuhlradEmil Reisler
Jan 27, 2005·Journal of Molecular Biology·V E GalkinEdward H Egelman
Dec 26, 2001·Biophysical Journal·Sofia Y Khaitlina, Hanna Strzelecka-Gołaszewska
Apr 10, 2012·Structure·Marissa G Saunders, Gregory A Voth
Sep 15, 2009·Structure·V E GalkinEdward H Egelman
Dec 19, 2001·Current Biology : CB·Edward H Egelman
Apr 18, 2014·Biophysical Journal·Marissa G SaundersGregory A Voth
Mar 8, 2002·Current Biology : CB·Natalya LukoyanovaEdward H Egelman
Jul 26, 2011·Current Biology : CB·Alphée Michelot, David G Drubin
Aug 9, 2006·Journal of Molecular Biology·Vadim A KlenchinIvan Rayment
Jun 30, 2007·Journal of Molecular Biology·John S AllinghamIvan Rayment
Sep 27, 2012·Biophysical Journal·Zeynep A Oztug DurerEmil Reisler
May 20, 2014·European Journal of Cell Biology·G HildBeáta Bugyi
Sep 26, 2003·Biophysical Journal·Roberto Dominguez, Philip Graceffa
Sep 26, 2003·Biophysical Journal·Dmitry S Kudryashov, Emil Reisler
Dec 6, 2005·Biophysical Journal·Victor F WaingehKathryn A Thomasson
Jun 19, 2007·Biophysical Journal·Markus BöhlHerwig O Gutzeit
Sep 2, 2008·Biophysical Journal·Zhiyong ZhangGregory A Voth
May 29, 2007·Biophysical Journal·Xiange ZhengDavid Sept
Feb 12, 2008·Biochemical and Biophysical Research Communications·József OrbánG Hild
Feb 14, 2016·Cytoskeleton·James R Bamburg, Barbara W Bernstein
Jul 12, 2008·Journal of Molecular Biology·Brannon R McCulloughEnrique M De la Cruz
Jul 18, 2006·Journal of Molecular Biology·Ying A WangEdward H Egelman
Oct 12, 2000·Biophysical Chemistry·E Kim, Emil Reisler
Dec 20, 2005·Biophysical Journal·Jhih-Wei Chu, Gregory A Voth
Feb 29, 2000·Biophysical Journal·A J Jin, R Nossal
Apr 17, 2002·Journal of Molecular Biology·Tomas NymanUno Lindberg
Jun 22, 2000·Journal of Molecular Biology·E KimEmil Reisler
Sep 8, 2001·Journal of Molecular Biology·A OrlovaEdward H Egelman
Mar 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ravine A Gungabissoon, James R Bamburg
Mar 12, 2010·Journal of Computational Chemistry·Sergei Grudinin, Stephane Redon
Feb 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Steven Z Chou, Thomas D Pollard
Aug 19, 2003·Nature Reviews. Molecular Cell Biology·Edward H Egelman
Aug 25, 2005·The Journal of Biological Chemistry·Melissa McKanePeter A Rubenstein
Jan 26, 2013·Biopolymers·Dmitri S Kudryashov, Emil Reisler
Jun 4, 2013·Nanoscale·Shivani SharmaJames K Gimzewski
Jun 19, 2003·The Journal of Biological Chemistry·Philip Graceffa, Roberto Dominguez
Aug 23, 2002·The Journal of Biological Chemistry·Kuo-Kuang WenPeter A Rubenstein
May 13, 2003·The Journal of Biological Chemistry·Takami Morita
Apr 30, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bader AlmuzzainiPiergiorgio Percipalle
Sep 10, 2004·The Journal of Biological Chemistry·Martine TourbezConstantin T Craescu
Jun 8, 2006·The Journal of Biological Chemistry·Andrea Pelikan ConchaudronMarie-France Carlier
Aug 22, 2006·The Journal of Biological Chemistry·Mark A RouldKathleen M Trybus
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Roberto Dominguez
Nov 13, 2004·The Journal of Biological Chemistry·Keith E Bryan, Peter A Rubenstein
Apr 29, 2005·The Journal of Biological Chemistry·Kuo-Kuang Wen, Peter A Rubenstein
Oct 30, 2007·The Journal of Biological Chemistry·Emil Reisler, Edward H Egelman
Sep 24, 2017·Scientific Reports·Esa-Pekka KumpulaInari Kursula
Sep 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Grażyna BudrynHoracio Pérez-Sánchez
Jun 24, 2018·Cancer Research·Hailong PeiTom K Hei
Jan 10, 2002·Biochemistry·Laurent Blanchoin, Thomas D Pollard

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