The ability of actin to both polymerize into filaments and to depolymerize permits the rapid rearrangements of actin structures that are essential for actin's function in most cellular processes. Filament polarity and dynamic properties are conferred by the hydrolysis of ATP on actin filaments. Release of inorganic phosphate (Pi) from filaments after ATP hydrolysis promotes depolymerization. We identify a yeast actin mutation, Val-159 to Asn, which uncouples Pi release from the conformational change that results in filament destabilization. Three-dimensional reconstructions of electron micrographs reveal a conformational difference between ADP-Pi filaments and ADP filaments and show that ADP V159N filaments resemble ADP-Pi wild-type filaments. Crystal structures of mammalian beta-actin in which the nucleotide binding cleft is in the "open" and "closed" states can be used to model actin filaments in the ADP and ADP-Pi conformations, respectively. We propose that these two conformations of G-actin may be related to two functional states of F-actin.
Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein
Direct evidence for ADP-Pi-F-actin as the major intermediate in ATP-actin polymerization. Rate of dissociation of Pi from actin filaments
Cytoplasmic concentrations of inorganic phosphate affect the critical concentration for assembly of actin in the presence of cytochalasin D or ADP
A mutation in an ATP-binding loop of Saccharomyces cerevisiae actin (S14A) causes a temperature-sensitive phenotype in vivo and in vitro.
Refinement of the F-actin model against X-ray fiber diffraction data by the use of a directed mutation algorithm
Localization of the tightly bound divalent-cation-dependent and nucleotide-dependent conformation changes in G-actin using limited proteolytic digestion
Conformational changes in subdomain 2 of G-actin: fluorescence probing by dansyl ethylenediamine attached to Gln-41
Binding of phosphate, aluminum fluoride, or beryllium fluoride to F-actin inhibits severing by gelsolin.
The sugar kinase/heat shock protein 70/actin superfamily: implications of conserved structure for mechanism
High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A
The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments
Dominant negative mutant actins identified in flightless Drosophila can be classified into three classes
Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation.
G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.
Mutant actins demonstrate a role for unpolymerized actin in control of transcription by serum response factor
A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast
In vivo and in vitro effects of two novel gamma-actin (ACTG1) mutations that cause DFNA20/26 hearing impairment
Defects in dynamics and functions of actin filament in Arabidopsis caused by the dominant-negative actin fiz1-induced fragmentation of actin filament
GTPase activity, structure, and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB
Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state
Water molecules in the nucleotide binding cleft of actin: effects on subunit conformation and implications for ATP hydrolysis
F-actin structure destabilization and DNase I binding loop: fluctuations mutational cross-linking and electron microscopy analysis of loop states and effects on F-actin
Nucleotide-mediated conformational changes of monomeric actin and Arp3 studied by molecular dynamics simulations
Nucleotide-dependence of G-actin conformation from multiple molecular dynamics simulations and observation of a putatively polymerization-competent superclosed state
Multiscale impact of nucleotides and cations on the conformational equilibrium, elasticity and rheology of actin filaments and crosslinked networks
Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex
The beta-thymosin/WH2 domain; structural basis for the switch from inhibition to promotion of actin assembly
The open nucleotide pocket of the profilin/actin x-ray structure is unstable and closes in the absence of profilin
Coarse-grained free energy functions for studying protein conformational changes: a double-well network model
Biochemical implications of a three-dimensional model of monomeric actin bound to magnesium-chelated ATP
Large-scale purification and in vitro characterization of the assembly of MreB from Leptospira interrogans
Cofilin binding to muscle and non-muscle actin filaments: isoform-dependent cooperative interactions
Cofilin increases the bending flexibility of actin filaments: implications for severing and cell mechanics
Polymerization, three-dimensional structure and mechanical properties of Ddictyostelium versus rabbit muscle actin filaments
The influence of divalent cations on the dynamic properties of actin filaments: a spectroscopic study
Actin cytoskeleton is required for nuclear accumulation of Gln3 in response to nitrogen limitation but not rapamycin treatment in Saccharomyces cerevisiae.
Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides
A mammalian actin substitution in yeast actin (H372R) causes a suppressible mitochondria/vacuole phenotype
INF2 Is a WASP homology 2 motif-containing formin that severs actin filaments and accelerates both polymerization and depolymerization
Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics
In β-actin knockouts, epigenetic reprogramming and rDNA transcription inactivation lead to growth and proliferation defects
Analysis of tetramethylrhodamine-labeled actin polymerization and interaction with actin regulatory proteins
Acceleration of yeast actin polymerization by yeast Arp2/3 complex does not require an Arp2/3-activating protein
Binding of Red Clover Isoflavones to Actin as A Potential Mechanism of Anti-Metastatic Activity Restricting the Migration of Cancer Cells
Structural Effects and Functional Implications of Phalloidin and Jasplakinolide Binding to Actin Filaments
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